PMID: 8948379Dec 1, 1996Paper

Correlation of genetic and serologic approaches to HIV-1 subtyping in Thailand

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
J GayweeS Nittayaphan

Abstract

The aim of this study was to compare the performance of differential polymerase chain reaction (PCR) typing and peptide enzyme-linked immunosorbent assay (V3-EIA) for human immunodeficiency virus type 1 (HIV-1) subtyping in Thailand using heteroduplex mobility assay (HMA) as the reference standard. Paired peripheral blood mononuclear cells (PBMC) and sera were collected from 38 HIV-1 seropositive persons in Thailand. HMA was done by standard methods; differential PCR employs primer pairs that differentially amplify either subtype E or B. V3-EIA used peptides specific for subtypes E or B. Thirty-two cases (84%) were found by HMA to be infected with subtype E: and six with (16%) subtype B. The results obtained with differential PCR were 100% concordant with those of HMA; V3 EIA correctly predicted the subtype in 95% (36 of 38). Six samples that molecularly subtyped as E were repeatedly dual reactive by screening V3-EIA, but these resolved to subtype E using an antigen-limiting EIA. Two samples were serologically nontypeable because of overall low levels of V3 antibody. Using HMA as the standard, differential PCR was shown to subtype HIV-1 reliably from patient PBMC samples. V3-EIA correctly predicted HIV-1 subtype in most (95%) o...Continue Reading

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Citations

Jun 16, 2001·AIDS·S TovanabutraJ K Carr
Oct 13, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·C J MasonS Nitayaphan
Dec 2, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Y M ChenH J Chang
Feb 3, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·T H NguyenH J Fleury
May 4, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J C PlantierF Barin
Sep 26, 2007·Retrovirology·Antoinette C van der Kuyl, Marion Cornelissen
Jan 11, 2007·International Journal of Medical Sciences·Bruce ShiramizuVictor Valcour
Jan 15, 2008·Journal of Neuroimmunology·Silvia Ratto-KimUNKNOWN Southeast Asia Research Collaboration with the University of Hawaii (SEARCH) 001 protocol team
Sep 3, 2013·Journal of Virological Methods·Raj Shankarappa, James I Mullins
Apr 30, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Veerachai WatanaveeradejSujitra Sukwit
Dec 31, 1998·AIDS Research and Human Retroviruses·C BeyrerK E Nelson
Jun 25, 1999·AIDS Research and Human Retroviruses·K Brengel-PesceJ M Seigneurin
Aug 12, 2003·AIDS Research and Human Retroviruses·Sodsai TovanabutraFrancine E McCutchan
Jun 10, 1997·AIDS Research and Human Retroviruses·S LouisirirotchanakulR Cheingsong-Popov
Oct 17, 2001·AIDS Research and Human Retroviruses·L García-AlbertUNKNOWN Group for the Study of Subtype Prevalence in Spain
Mar 10, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Y M ChenY C Lan
Aug 1, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·C BeyrerC Natpratan

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