PMID: 3771732Sep 1, 1986Paper

Correlation of HBV DNA and monoclonal reactivity to HBsAg in serum of patients with HBV infection

Journal of Virological Methods
J B ZeldisJ Wands

Abstract

Hepatitis B virus (HBV) DNA hybridization assay, a monoclonal radioimmunoassay (M-RIA) for hepatitis B surface antigen (HBsAg) and conventional polyclonal immunoassays for HBV associated antigens were used to study sera from patients on dialysis and with acute hepatitis B. HBV DNA was detectable in hepatitis B e antigen (HBeAg) negative patients with acute hepatitis but not in HBsAg+ HBeAg- dialysis patients. In acute hepatitis, HBsAg immunoreactivity by M-RIA could still be detected even though a commercial immunoassay for HBsAg, the AUSRIA II, and the HBV DNA assay were no longer positive. Unlike in acute HBV infection, serum HBV DNA was detectable in dialysis patients who were AUSTRIA II negative but M-RIA positive. Serial determination of HBsAg by M-RIA and HBV DNA revealed episodes of HBV DNA positivity months after both the HBsAg was no longer positive by polyclonal immunoassay. Thus, the M-RIA for HBsAg and the molecular hybridization technique for HBV DNA are sensitive and specific assays for the identification of potentially infectious individuals who would not have been characterized as such based on the results of conventional polyclonal immunoassays.

References

May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A FigusA Balestrieri
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E Ben-PorathK Isselbacher
May 1, 1984·Journal of Virological Methods·S V FeinmanJ E Maynard
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W KamW J Rutter
Jan 1, 1982·Journal of Medical Virology·M BerningerJ L Gerin

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Citations

Oct 1, 1989·The Journal of Clinical Investigation·T J LiangJ R Wands
May 1, 1989·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·K N LaiH J Lin
Jul 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J B ZeldisH N Steinberg
Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T J LiangJ R Wands
Jun 1, 1990·Journal of Virology·H S SteinbergJ B Zeldis

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