PMID: 2499357Jun 15, 1989Paper

Correlation of intracellular and extracellular calcium ion concentrations with synergy between 1,2-dioctanoyl-sn-glycerol and ionomycin in platelet arachidonic acid mobilization

Biochimica Et Biophysica Acta
Y OzakiS Kume

Abstract

The potentiation by 1,2-dioctanoyl-sn-glycerol (DiC8) of ionomycin-induced platelet production of 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) was investigated in correlation with extracellular Ca2+ concentrations and increases in [Ca2+]i, as detected with aequorin and fura-2. Extracellular Ca2+ concentrations greatly influenced the production of arachidonic acid metabolites induced by DiC8 and ionomycin, while that induced by ionomycin alone was minimally affected by variation of the extracellular Ca2+ concentration. In the synergy between ionomycin and 20 microM DiC8, the optimal concentrations of ionomycin shifted from high to low with increasing concentrations of extracellular Ca2+, suggesting that there might be a range of optimal [Ca2+]i for the production of the arachidonic acid metabolites. This hypothesis was confirmed by simultaneous measurements of [Ca2+]i increases, and the production of the arachidonic acid metabolites. With the aequorin method, the optimal concentrations of [Ca2+]i fell to between 10 microM and 20 microM, and with the fura-2 method, it fell to between 800 nM and 1800 nM. Direct measurements of [14C]arachidonic acid release suggested that t...Continue Reading

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Citations

Jan 15, 1993·European Journal of Pharmacology·O LjunggrenU H Lerner
Dec 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T M el Attar, H S Lin
Jan 1, 1989·Progress in Lipid Research·H AritaK Hanasaki
Oct 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·W SakamotoS Murota
Mar 16, 1992·Biochemical and Biophysical Research Communications·Y OzakiS Kume

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