Correlation of long-range membrane order with temperature-dependent growth characteristics of parent and a cold-sensitive, branched-chain-fatty-acid-deficient mutant of Listeria monocytogenes

Archives of Microbiology
Scott L JonesPhilip D II Morse

Abstract

Listeria monocytogenes is a food-borne, pathogenic, psychrotolerant bacterium that grows at refrigeration temperatures. Long-range membrane order of the parent (10403S) and of a cold-sensitive mutant ( cld-1) deficient in odd-numbered, branched-chain fatty acids was measured using the width of the central line of spectra of an electron paramagnetic resonance probe, 4,4-dimethyl-2-heptyl-2-hexyloxazolidine- N-oxyl (7N14), that locates deep in the hydrocarbon region of the membranes. The line width decreased from 0.9 to 0.5 milliTesla (mT) over the temperature range of 0-10 degrees for strain 10403S and -5 to 32 degrees C for strain cld-1 independent of protein state (heat denatured or intact). This provided new evidence for phase transitions in the membranes. When strain cld-1 was grown in medium supplemented with 2-methylbutyric acid, which restores anteiso fatty acids and the ability to grow at low temperature, the change in central line width as a function of temperature resembled that of strain 10403S. The temperatures at which the central line width became 0.8 mT corresponded to those at which growth became very slow in both strains (3-5 degrees C for 10403S, 15 degrees C for cld-1) as determined by Arrhenius plots. These d...Continue Reading

Citations

Jun 12, 2003·Journal of Biosciences·M K Chattopadhyay, M V Jagannadham
Aug 21, 2007·Applied and Environmental Microbiology·Mohamed Badaoui NajjarThomas J Montville
Mar 28, 2002·Applied and Environmental Microbiology·Siqing LiuBrian J Wilkinson
Dec 9, 2003·Applied and Environmental Microbiology·Apostolos S Angelidis, Gary M Smith
Jul 31, 2012·Journal of Bacteriology·Yvonne SunMary X D O'Riordan
Jul 18, 2006·Chemistry and Physics of Lipids·Andrea SchreiberVolker Thiel
Jul 4, 2003·Science Progress·Michael H Weber, Mohamed A Marahiel
Aug 14, 2020·Genes·Ashley M EdwardsPatricia C Dos Santos
Apr 20, 2021·Archives of Microbiology·Alexander FleglerAndré Lipski
May 19, 2007·Analytical Chemistry·Saskia ThurnhoferWalter Vetter

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