PMID: 3753597Jan 15, 1986Paper

Correlation of macrophage-mediated tumor-cell lysis with the production of macrophage cytolytic factor (CF). Preliminary characterization of a factor inhibiting CF production

International Journal of Cancer. Journal International Du Cancer
G E GiffordR Gallily

Abstract

Macrophage-mediated cytolysis of thymidine-prelabelled murine A9 fibrosarcoma cells was compared to the level of cytolytic factor (CF) present in the cultures by assaying supernatant aliquots on actinomycin (AcD)-treated A9 fibrosarcoma cells. A good correlation between the level of A9 killing and CF titer was observed when different concentrations of lipopolysaccharide (LPS) were added to various macrophage populations: murine peritoneal cells, short-term bone-marrow (BM)-derived macrophages and JBM phi macrophage lines. Optimal A9 killing and CF secretion, equivalent to the killing of about 1000 AcD-pretreated A9 cells by a single macrophage, were obtained following activation of JBM phi by LPS. CF production by BM-derived macrophages was enhanced in serum-free medium when compared to its release in the presence of fetal calf serum. The LPS-activated macrophages could be restimulated by the activating agent to produce CF following one week of propagation in the absence of LPS. On the other hand, CF activity was absent from the supernatants of activated macrophages co-cultured with normal embryonic fibroblasts, which are resistant to macrophage-mediated killing. This effect could be attributed to a factor, secreted by normal f...Continue Reading

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Citations

Dec 1, 1992·Biulleten' eksperimental'noĭ biologii i meditsiny·A E MedvedevA E Zemliakov
Apr 15, 1987·Klinische Wochenschrift·R Munker, H P Koeffler
Jan 1, 1991·Biotherapy·G E Gifford, D H Duckworth
Jan 7, 2017·Oncotarget·Huafeng WangLinjing Zhang
Jan 1, 1989·Seminars in Surgical Oncology·H J Wanebo
Apr 20, 2018·Frontiers in Cell and Developmental Biology·Luca Cassetta, Takanori Kitamura

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