Correlation of neuronal loss with increased expression of NADPH diaphorase in cultured rat cerebellum and cerebral cortex

Brain Research
G K Page, A J Morton

Abstract

NADPH diaphorase expression in neurones and glial cells was examined in primary cultures of embryonic cerebellum and cerebral cortex with (i) increasing age in culture and (ii) the exogenous application of glutamate. In neurone-enriched cultures from both regions, NADPH diaphorase histochemistry selectively labelled discrete sub-populations of neurones and glial cells. Double labelling of the cultures showed that 2-4% of the cells with a neuronal phenotype were NADPH diaphorase-positive. Although the total numbers of neurones present in the cultures declined with increased age of cultures, there was no change in the percentage of NADPH diaphorase-positive neurones with time. In contrast, the percentage of NADPH diaphorase-positive glial cells increased from around 10% at 7 days in culture to more than 50% after 3 or more weeks in both cortical and cerebellar cultures. The age-related increase in staining was due to a greater number of cells expressing NADPH diaphorase activity rather than increased activity of existing enzyme. There was a strong correlation between the decline in neuronal cell population and the increase in the number of NADPH diaphorase positive glial cells. To determine whether or not there was a relationship...Continue Reading

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Citations

Jun 26, 2010·BMC Neuroscience·Santos BlancoMaria A Peinado

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