PMID: 7536835Jan 1, 1995Paper

Correlation of serum concentrations of PSA-ACT complex with total PSA in random and serial specimens from patients with BPH and prostate cancer

Journal of Clinical Laboratory Analysis
J T WuR Stephenson

Abstract

The current assays for serum prostate specific antigen (PSA) have failed to produce the same PSA values on the same specimens because of problems with antibody specificity and calibrator preparation. To eliminate these problems, we proposed to replace the current serum PSA assay with an assay specific for the PSA-ACT (PSA-alpha 1-antichymotrypsin) complex in the serum. An assay specific for the PSA-ACT complex was established using the anti-PSA antibody to coat the microplate for capturing the PSA complex and anti-ACT polyclonal antibodies for quantification. There was an excellent correlation between serum concentrations of PSA-ACT and total PSA, using either the Hybritech calibrator (gamma = 0.996) or a serum calibrator prepared in house (gamma = 0.993), in random as well as in serial specimens from 14 individual patients. Even though we did not find a gradual increase in the percentage of PSA-ACT with the increase of total PSA in cancer patients, a slightly higher percentage of free PSA was measured in pooled normal sera (18%) and in pooled sera containing only 12 ng/ml of total PSA (12%), compared to serum pools containing elevated PSA (> 100 ng/ml) level, in which most PSA was in the complex form (95%). Therefore, using an...Continue Reading

Citations

Dec 16, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·F EspañaJ F Jiménez-Cruz
Apr 16, 1998·International Journal of Urology : Official Journal of the Japanese Urological Association·M KuriyamaJ Shimazaki
Jul 10, 2007·Analytical Biochemistry·Ziad J SahabQing-Xiang Amy Sang
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin
Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T WuR Stephenson
Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T Wu, L W Wilson
Sep 17, 2004·International Reviews of Immunology·Veljko VeljkovicRadmila Metlas
Mar 17, 2000·The Journal of Clinical Endocrinology and Metabolism·A BansalA W Meikle

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