Correlation of survivin, p53 and Ki-67 in laryngeal cancer Hep-2 cell proliferation and invasion

Asian Pacific Journal of Tropical Medicine
Shi-Geng PeiHong Zhang

Abstract

To investigate the mechanism of survivin, p53 and Ki-67 on Hep-2 human laryngeal cancer endothelial cell proliferation and invasion. Laryngeal squamous cell carcinoma and paracancerous normal tissues were collected, total RNA was extracted from tissues, survivin, p53 and Ki-67 gene mRNA expression levels in laryngeal cancer and the adjacent tissues were detected by Real-time PCR. Human laryngeal cancer Hep-2 epithelial cells were selected, survivin gene was overexpressed, and cell proliferation was detected by MTT. p53 and Ki-67 gene expression changes in overexpressed survivin gene were detected by Western blot. Changes in Hep-2 cell invasive ability were studied when survivin was overexpressed as detected by Transwell invasion assay. In the adjacent tissues, survivin, p53 and Ki-67 gene relative expression levels were 1.72 ± 0.9, 13.7 ± 5.7 and 5.7 ± 1.3, respectively; while in cancer tissues, gene relative expression levels were 53.7 ± 8.3, 66.7 ± 5.2 and 61.0 ± 3.1, respectively, which was significantly increased. As detected by MTT, relative cell survival rate within 12 h of survivin overexpression were: load control group (88.5 ± 1.6)%; overexpressed group (90.3 ± 1.9)%. Transwell invasion assay results indicated that ove...Continue Reading

References

Dec 5, 1998·Oncogene·E SchneiderP Wagner
Oct 6, 2005·Journal of Cellular Physiology·Jörn BullwinkelThomas Scholzen
Apr 20, 2006·Cancer Letters·N K SahP S Bisen
Dec 15, 2010·Clinical Medicine Insights. Pathology·Mari Markkanen-LeppänenJaana Hagström
Apr 13, 2011·Cancer Letters·Valentina MirisolaAngelo Salami
Jul 17, 2014·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z-G ShiG Liu
Sep 18, 2014·Therapeutics and Clinical Risk Management·Susanna Hilda HutajuluJaap M Middeldorp

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Citations

Mar 19, 2020·Journal of Clinical Medicine·Yonghwan ShinKyungpyo Park
Dec 7, 2018·Bioorganic Chemistry·Mohammad Shahidul IslamAssem Barakat

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