Correlation of the activation of Ca2+/calmodulin-dependent protein kinase II with the initiation of insulin secretion from perifused pancreatic islets

Endocrinology
R A EasomM Landt

Abstract

An experimental procedure has been designed to permit the simultaneous assessment of the activation status of the multifunctional Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) with insulin secretion in perifused islets. By this procedure, the activation of CaM kinase II by glucose correlated closely with the initial and sustained phases of insulin secretion within a 30-min test period. By contrast, islets (160-200/tube) in static incubations neither supported second-phase insulin secretion nor CaM kinase II activation beyond 10-15 min. This was not the result of the accumulation of insulin, because the introduction of insulin (40-160 ng/ml) into the perifusion medium failed to mimic the suppression of glucose-induced insulin secretion or CaM kinase II activation. A similar addition of SRIF (0.01-1 microM) or epinephrine (1 microM) profoundly suppressed insulin secretion although failing to significantly influence CaM kinase II activation. Finally, on withdrawal of glucose from perifused islets, insulin secretion rapidly returned to basal rates, but CaM kinase II deactivation was significantly delayed. The correlation of kinase activation with the initiation of insulin secretion suggests that CaM kinase II may be i...Continue Reading

References

Jan 1, 1979·Endocrinology·G SomersW J Malaisse
Jun 30, 1975·Annals of the New York Academy of Sciences·W J MalaisseL Orci
Nov 30, 1992·Biochemical and Biophysical Research Communications·R M WenhamR A Easom
Aug 31, 1990·Biochemical and Biophysical Research Communications·J L WangM L McDaniel
Jan 1, 1989·Progress in Biophysics and Molecular Biology·F M Ashcroft, P Rorsman
Nov 1, 1989·Endocrinology·W S ZawalichH Rasmussen
Oct 1, 1987·Physiological Reviews·M Prentki, F M Matschinsky
Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Feb 1, 1993·The Biochemical Journal·S J HughesS J Ashcroft

❮ Previous
Next ❯

Citations

Oct 20, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kirsten I LarsenJohn P Williams
May 9, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L M MatovcikF S Gorelick
May 12, 1998·The American Journal of Physiology·P M Jones, S J Persaud
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C H ChangT M Chang
Jan 16, 1999·The Journal of Biological Chemistry·K MatsumotoE Miyamoto
Sep 1, 2000·Seminars in Cell & Developmental Biology·R A Easom
Oct 8, 2009·Cell Metabolism·Ludmila V DeriyDeborah J Nelson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.