PMID: 11336344May 5, 2001Paper

Correlation of the capacity factor in vesicular electrokinetic chromatography with the octanol:water partition coefficient for charged and neutral analytes

Pharmaceutical Research
J L RazakC E Lunte

Abstract

The aim of this study was to develop a method based upon electrokinetic chromatography (EKC) using oppositely charged surfactant vesicles as a buffer modifier to estimate hydrophobicity (log P) for a range of neutral and charged compounds. Vesicles were formed from cetyltrimethylammonium bromide (CTAB) and sodium n-octyl sulfate (SOS). The size and polydispersity of the vesicles were characterized by electron microscopy, dynamic light scattering, and pulsed-field gradient NMR (PFG-NMR). PFG-NMR was also used to determine if ion-pairing between cationic analytes and free SOS monomer occurred. The CTAB/SOS vesicles were used as a buffer modifier in capillary electrophoresis (CE). The capacity factor (log k') was calculated by determining the mobility of the analytes both in the presence and absence of vesicles. Log k' was determined for 29 neutral and charged analytes. RESULTS; There was a linear relationship between the log of capacity factor (log k') and octanol/water partition coefficient (log P) for both neutral and basic species at pH 6.0, 7.3, and 10.2. This indicated that interaction between the cation and vesicle was dominated by hydrophobic forces. At pH 4.3, the log k' values for the least hydrophobic basic analytes wer...Continue Reading

Citations

May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
Feb 3, 2009·Analytical and Bioanalytical Chemistry·Yveline HenchozSophie Martel
Sep 15, 2005·Analytical Chemistry·Tetsuo OkadaTomoo Kido

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