Correlation of the detection of Clostridium difficile toxins in stools and presence of the clostridia in tissues of children

Human Pathology
Jeannette GuarnerSherif R Zaki

Abstract

Clostridium difficile toxin is frequently found in the stool of children; however, pseudomembranous colitis is rare. Studying the usefulness of Clostridium difficile toxin assays in pediatrics is required. We performed a correlation between presence of Clostridium difficile toxin in stool and evidence of Clostridium difficile in gastrointestinal pediatric tissue samples using immunohistochemistry (with a pan-clostridial antibody) and polymerase chain reaction (with primers for toxin genes). We studied 11 patients with a median age of 8 years (range, 4 weeks to 17 years); 4 (36%) were female. The median time between detection of Clostridium difficile toxin in stool and obtaining tissue was 3 days. Ten patients survived. Endoscopy was performed in 8 survivors and showed normal mucosa in 2, pseudomembranes in 2, erythema and friability in 1, aphthae in 1, increased mucous production in 1, and colitis in 1. Two survivors underwent laparotomy for either obstruction or resection of necrotic bowel. Histopathologic studies in these 10 surviving patients showed necrosis in 2 samples, granulomatous inflammation in 1, moderate colitis in 1, and mild to minimal pathology in 7. There was no antigenic or molecular evidence of clostridia in t...Continue Reading

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Citations

Dec 22, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jeannette Guarner, Colleen S Kraft
Jun 15, 2013·Current Problems in Surgery·J D StanleyJ H Ashcraft
Jun 15, 2011·The Journal of Infection·David A EnochJ Andreas Karas
Jul 5, 2015·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Elena BoraliCostantino De Giacomo
May 13, 2014·Seminars in Diagnostic Pathology·Georgina UbertiDaniela S Allende

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