Correlation of the predisposition of Chinese children to cerebral palsy with nucleotide variation in pri-miR-124 that alters the non-canonical apoptosis pathway

Acta Pharmacologica Sinica
Hui LiAi-Min Li

Abstract

Cerebral palsy is a group of non-progressive motor impairment syndromes caused by brain lesions during development. Herein, we investigated the relationship between nucleotide variations in a miRNA coding region and the predisposition of Chinese children to cerebral palsy. A total of 233 CP patients and 256 healthy participants were enrolled, and 60 children were selected from each group for plasma miRNA detection. We screened the coding regions of pri-miR-124-1, -2, and -3 using PCR and sequencing. The expression of miR-124 was determined by qRT-PCR. Luciferase assays and Western blots were used to confirm the regulation of target genes by miR-124. The function of miR-124 was further identified in SH-SY5Y cells by detecting cell viability and apoptosis. We revealed that the rare alleles T of rs3802169 and G of rs191727850 were found to be associated with an increased risk of cerebral palsy (OR=3.71, 95% CI 1.74-7.92 and OR=2.18, 95% CI 1.36-3.49, respectively). We further showed that the levels of mature miR-124 were down-regulated by the C-to-T variation in vitro. More importantly, the reduction of miR-124 resulting from the C-to-T change led to the less-efficient inhibition of the target genes ITGB1, LAMC1 and BECN1, which m...Continue Reading

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Citations

Jun 5, 2020·Artificial Cells, Nanomedicine, and Biotechnology·Dan JiQiong Fang
Jun 17, 2020·Developmental Medicine and Child Neurology·Ryan PhamJesia G Berry

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Methods Mentioned

BETA
PCR
Assay
flow cytometry
FACS
enzyme-linked immunosorbent assay
transfection
ELISA
nuclear translocation

Software Mentioned

SPSS Package
DNAMAN
AlphaView SA
BLAST
SHEsis
TargetScan

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