Correlations between function and dynamics: time scale coincidence for ion translocation and molecular dynamics in the gramicidin channel backbone

Biochemistry
C L North, T A Cross

Abstract

The polypeptide backbone of gramicidin lines the channel lumen, which maintains a single file string of water molecules and the occasional cation. Whether or not local motions of the backbone are important for facilitating cation transport has been the subject of debate. Here it is demonstrated that motions of the backbone occur on the same time scale as cation translation through this transmembrane channel. To characterize the frequency of the local motions, field-dependent 15N T1 relaxation times were interpreted in light of an independently determined motional model that defines the motions as occurring about the C alpha-C alpha axis. To overdamp these local motions, several models that involve extensive correlations of the molecular motions were considered. One of these schemes for correlated motions suggests a unidirectional reaction path that would minimize the time necessary for the cation to pass through the low-dielectric center of the bilayer.

Citations

Dec 31, 1997·Solid State Nuclear Magnetic Resonance·M CottenT A Cross
Dec 17, 2002·Journal of Magnetic Resonance·Riqiang FuTimothy A Cross
Dec 2, 2004·The Journal of General Physiology·Toby W AllenBenoit Roux
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·R Fu, T A Cross
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·F KovacsT A Cross
Feb 10, 2016·Molecular Membrane Biology·Simon G Patching
Dec 25, 2007·Magnetic Resonance in Chemistry : MRC·N R Skrynnikov
Feb 10, 2010·Biophysical Journal·Martin KurylowiczRégis Pomès
Jan 15, 2014·Journal of Magnetic Resonance·Timothy A CrossAnna Wright
Apr 23, 2009·Biophysical Journal·Santi Esteban-MartínJesús Salgado
Nov 4, 1998·Journal of Magnetic Resonance·F TianT A Cross
Feb 2, 1999·Journal of Molecular Biology·F Tian, T A Cross
Jul 22, 2005·Journal of Biomolecular NMR·Dustin W BleileSuzana K Straus
Oct 2, 2007·Journal of the American Chemical Society·Veniamin ChevelkovNikolai R Skrynnikov
Jul 27, 2001·Journal of the American Chemical Society·S KimT A Cross

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