Correlations between in vivo and in vitro effects of toxic compounds: Studies with hydrazine

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C J WaterfieldJ A Timbrell

Abstract

Studies have been carried out in rats in vivo and in isolated hepatocytes from the same strain of rat in vitro using the hepatotoxicant hydrazine as a model compound. These studies have shown that a number of biochemical changes occur and are measurable in both systems. However, despite measuring the same parameters in each system, the effects do not necessarily show a quantitative or qualitative correlation. Thus depletion of glutathione and ATP occurred in both systems but required a much higher concentration in vitro. The effects on more liver-specific parameters such as triglyceride, citrulline and taurine levels in vivo were different or not observed in vitro and the inhibition of urea synthesis and cytotoxicity in vitro were not observed in vivo, although these endpoints are more relevant markers of hepatic effects. Inhibition of protein synthesis proved to be the marker that showed the best correlation, occurring at a similar concentration in vitro as in vivo, although not to the same extent. The importance of identifying specific endpoints of toxicity, the problems of comparing in vivo with in vitro data and the limitations in their interpretation are highlighted by the data presented. A knowledge of the underlying mech...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·P MoldéusS Orrenius
Jan 1, 1992·Archives of Toxicology·S GhatinehJ A Timbrell
Mar 1, 1992·Human & Experimental Toxicology·N E PreeceJ A Timbrell
Jan 1, 1990·Archives of Toxicology·N E PreeceJ A Timbrell
Mar 1, 1989·Toxicology and Applied Pharmacology·D DeboyserM Roberfroid
Sep 1, 1971·Biochemical Pharmacology·A RobergeR Charbonneau
May 1, 1970·Biochemical Pharmacology·D A ClarkD L Trout
May 1, 1965·Biochemical Pharmacology·D L Trout
Apr 1, 1983·Journal of Toxicology and Environmental Health·D L StoryD H Gould
Dec 1, 1982·Journal of Toxicology and Environmental Health·J A TimbrellA J Streeter
Jul 1, 1995·European Journal of Pharmacology·C J WaterfieldJ A Timbrell
Dec 1, 1993·Toxicology and Applied Pharmacology·M A CarfagnaG L Kedderis
Dec 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J Delaney, J A Timbrell
Sep 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P D WorboysJ B Houston
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Dec 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·X PonsodaM J Gómez-Lechón
Feb 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R JoverM J Gómez-Lechón
Jun 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Ghatineh, J A Timbrell
Jan 1, 1996·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·C J WaterfieldJ A Timbrell

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Citations

Feb 4, 1998·Chemico-biological Interactions·C J WaterfieldJ A Timbrell
May 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C DilworthJ A Timbrell
Nov 2, 2005·Phytotherapy Research : PTR·Negussu MekonnenJohn Timbrell
May 2, 2014·Journal of Gastroenterology and Hepatology·Urs A Boelsterli, Kang Kwang Lee
Jul 7, 2007·Drug and Chemical Toxicology·P VivekanandanS Karthikeyan
Sep 17, 2004·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·T G KlenøU G Sidelmann
Sep 11, 2004·Assay and Drug Development Technologies·Yuansheng Yang, R Robert Balcarcel
Jun 9, 2004·International Journal of Occupational Safety and Ergonomics : JOSE·Jolanta Skowroń, Lidia Zapór
Mar 3, 2004·BioTechniques·Yuansheng Yang, R Robert Balcarcel
Apr 29, 2010·Journal of Proteome Research·Kirill A VeselkovJeremy K Nicholson

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