Correlations between synthetic peptide-based enzyme immunoassays and immunofluorescence assay for detection of human herpesvirus 8 antibodies in different Argentine populations

Journal of Medical Virology
Celeste PérezJorge Gómez

Abstract

Human herpesvirus 8 (HHV-8) antibody tests vary in sensitivity and specificity, depending on the population tested and on the type of assay. In this study, we evaluated the sensitivity and specificity of two peptide enzyme immunoassays using a multiple antigenic peptide (PK8.1-MAP) or a chimeric peptide (PK8.1-orf65) as the antigens and determined the HHV-8 seroprevalence in different Argentine populations using an immunofluorescence assay (IFA) as reference. For analysis, when either or both of the peptide EIAs were positive, the specimen was considered positive (PEIA). We estimated the sensitivity and specificity of PEIA to be 97% using Kaposi's sarcoma (KS) patients and healthy individuals as positive and negative controls respectively. Then, we expanded the control groups to include IFA positive men who have sex with men (MSM) and IFA negative blood donors. The sensitivity decreased to 83% but specificity remained high at 98%. Concordance between PEIA and IFA was 77% for 1/40 IFA titers and increased to 90% for titers >or=1/160. Seroprevalences for HHV-8 performed in the HIV positive MSM were (IFA 73.1%; PEIA55.2%); heterosexuals (52.5%, 22.2%), which includes injecting drug users (IDU) (54.0%, 32.4%) and non-IDU (51.6%, 16...Continue Reading

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Citations

Nov 8, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Juan V GuaniraUNKNOWN Peruvian HIV Sentinel Surveillance Working Group
Dec 25, 2009·Emerging Infectious Diseases·Celeste L PérezSofía Camino
Feb 14, 2014·Systematic Reviews·Eliane RohnerJulia Bohlius
Aug 10, 2016·Journal of Medical Virology·María Laura HulaniukMariela Caputo

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