Correlations between the Composition of the Bovine Microbiota and Vitamin B12 Abundance

MSystems
Julian Franco-LopezJennifer Ronholm

Abstract

Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows-and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in determining what causes vitamin B12 variability. We collected 92 temporally linked rumen, fecal, blood, and milk sample sets from Holstein cows at various stages of lactation fitted with rumen cannula and attempted to define which bacterial genera correlated well with vitamin B12 abundance. The level of vitamin B12 present in each sample was measured, and the bacterial population of each rumen, fecal, and milk sample (n = 263) was analyzed by 16S rRNA-targeted amplicon sequencing of the V4 region. The bacterial populations present in the rumen, small intestine, and milk were highly dissimilar. Combined diet and lactation status had significant effects on the composition of the microbiota in the rumen and in the feces. A high ruminal concentration of vitamin B12 was correlated with the increased abundance of Prevotella, while a low ruminal concentration of vitamin B12 was correlated with increased abundance of Bacteroidetes, Ruminiclostridium, ...Continue Reading

References

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Citations

Jun 3, 2020·Methods : a Companion to Methods in Enzymology·C L Girard, B Graulet
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Mélissa DuplessisJennifer Ronholm

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Datasets Mentioned

BETA
PRJNA527029

Methods Mentioned

BETA
amplicon sequencing
PCR

Software Mentioned

MicrobiomeAnalyst
PERMANOVA
R Vegan
SAS
ANOSIM
LASSO
NMDS
mothur
Marker Data Profiling ( MDP )
LEfSe

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