Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context

Nanoscale
Kerda KeevendInge K Herrmann

Abstract

The understanding of living systems and their building blocks relies heavily on the assessment of structure-function relationships at the nanoscale. Ever since the development of the first optical microscope, the reliance of scientists across disciplines on microscopy has increased. The development of the first electron microscope and with it the access to information at the nanoscale has prompted numerous disruptive discoveries. While fluorescence imaging allows identification of specific entities based on the labelling with fluorophores, the unlabelled constituents of the samples remain invisible. In electron microscopy on the other hand, structures can be comprehensively visualized based on their distinct electron density and geometry. Although electron microscopy is a powerful tool, it does not implicitly provide information on the location and activity of specific organic molecules. While correlative light and electron microscopy techniques have attempted to unify the two modalities, the resolution mismatch between the two data sets poses major challenges. Recent developments in optical super resolution microscopy enable high resolution correlative light and electron microscopy, however, with considerable constraints due t...Continue Reading

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Methods Mentioned

BETA
light microscopy
fluorescence microscopy
traditional
electron microscopy
electron
super resolution microscopy
electron beam
X-ray
super-resolution microscopy

Software Mentioned

CSREM
MINIFLUX
CCLEM
CLAIRE
ColorEM

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