PMID: 11918850Mar 29, 2002Paper

Correlative changes of interferon-gamma and interleukin-4 between cortical layer and pulmonary airway of sensitized rats

Acta Pharmacologica Sinica
Qiang-Min XieRu-Lian Bian

Abstract

To explore correlation of T helper 1 (Th1) and Th2 cytokine between the cortical layer and pulmonary airway in ovalbumin-induced rat asthma model. Aerosol antigen-induced changes of inflammation in bronchoalveolar lavage fluids (BALF) and pulmonary histologic section in sensitized rats were investigated. Changes of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in BALF and cortical layer homogenates were determined by ELISA. The number of inflammatory cells in BALF in antigen challenged group was significantly higher than control group (P <0.05). Dexamethasone (DXM, 0.5 mg/kg, ip) markedly reduce total leukocyte numbers in BALF, and almost completely inhibited eosinophil, lymphocyte accumulation. The score of histological examination (eosinophil infiltration, mucous edema and epithelial lesion) in antigen challenged group was significantly higher than control group (P <0.05). DXM (0.5 mg/kg, ip) reduced the numbers of eosinophils and improved mucous edema and epithelial lesion of bronchi and bronchioles. In addition, our results demonstrated decreased IFN-gamma level accompanied by increased IL-4 level that resulted in a decreased IFN-gamma/IL-4 ratio in the BALF after the sensitized rats were challenged with aerosol ant...Continue Reading

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