Correlative cryogenic tomography of cells using light and soft x-rays

Ultramicroscopy
Elizabeth A SmithCarolyn A Larabell

Abstract

Correlated imaging is the process of imaging a specimen with two complementary modalities, and then combining the two data sets to create a highly informative, composite view. A recent implementation of this concept has been the combination of soft x-ray tomography (SXT) with fluorescence cryogenic microscopy (FCM). SXT-FCM is used to visualize cells that are held in a near-native, cryopreserved. The resultant images are, therefore, highly representative of both the cellular architecture and molecular organization in vivo. SXT quantitatively visualizes the cell and sub-cellular structures; FCM images the spatial distribution of fluorescently labeled molecules. Here, we review the characteristics of SXT-FCM, and briefly discuss how this method compares with existing correlative imaging techniques. We also describe how the incorporation of a cryo-rotation stage into a cryogenic fluorescence microscope allows acquisition of fluorescence cryogenic tomography (FCT) data. FCT is optimally suited for correlation with SXT, since both techniques image the specimen in 3-D, potentially with similar, isotropic spatial resolution.

References

Dec 1, 1976·Journal of Clinical Ultrasound : JCU·L Badria, G B Young
Dec 1, 1988·Journal of Virological Methods·Y Hosaka, T Watabe
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
Mar 12, 1999·Science·W E Moerner, M Orrit
Mar 24, 1999·Trends in Cell Biology·W BaumeisterJ Walz
Sep 11, 1999·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·W A WeberM Schwaiger
Mar 22, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Takizawa, J M Robinson
Mar 10, 2001·Journal of Microscopy·W Meyer-IlseC A Larabell
May 10, 2002·Journal of Microscopy·R Heintzmann, C Cremer
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jianwei MiaoYoshinori Nishino
Feb 1, 2005·FEBS Letters·Roger Y Tsien
Jul 1, 2005·Nature·Weilun ChaoDavid T Attwood
Oct 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·David ShapiroDavid Sayre
Nov 10, 2005·Nature Methods·Markus GrabenbauerTommy Nilsson
Nov 22, 2005·Nature Methods·Nathan C ShanerRoger Y Tsien
Apr 15, 2006·Science·Ben N G GiepmansRoger Y Tsien
Sep 22, 2007·Journal of Structural Biology·Anna SartoriJuergen M Plitzko
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Peter M Carlton
Jun 21, 2008·Histochemistry and Cell Biology·Vladan LucićWolfgang Baumeister
Jul 2, 2009·Journal of Microscopy·M A Le GrosC A Larabell
Oct 13, 2009·Trends in Cell Biology·Gerry McDermottCarolyn A Larabell
Aug 1, 1976·Applied Optics·B NiemannG Schmahl
Feb 25, 2010·Journal of Structural Biology·Alexander RigortJürgen M Plitzko
Apr 7, 2010·Physical Review Letters·Xiaojing HuangChris Jacobsen
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Johanna NelsonChris Jacobsen
Sep 28, 2010·Current Opinion in Structural Biology·Carolyn A Larabell, Keith A Nugent
Nov 26, 2010·Nature Methods·Shigeki WatanabeErik M Jorgensen
Dec 24, 2010·Journal of Microscopy·D VanheckeV Lučić
Jul 26, 2011·Current Opinion in Structural Biology·Jeffrey CaplanKirk J Czymmek
Sep 13, 2011·Journal of Structural Biology·Gavin E MurphySriram Subramaniam
Nov 29, 2011·Journal of Structural Biology·H M HertzS Svärd
Dec 14, 2011·Journal of Structural Biology·Waltraud G MüllerJames G McNally
Dec 20, 2011·Journal of Structural Biology·Francisco Javier ChichónJosé L Carrascosa
Jan 3, 2012·Journal of Structural Biology·Christoph HagenKay Grünewald
Jan 17, 2012·Annual Review of Physical Chemistry·Gerry McDermottCarolyn A Larabell
Feb 1, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gerry McDermottCarolyn A Larabell
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G KopekHarald F Hess
Jun 23, 2012·Nature Reviews. Molecular Cell Biology·Karolin LugerDavid J Tremethick
Aug 24, 2013·Journal of Cellular Biochemistry·Bertrand P CinquinCarolyn A Larabell

❮ Previous
Next ❯

Citations

Nov 25, 2014·Biophysical Journal·Elizabeth A SmithCarolyn A Larabell
Jun 15, 2015·Archives of Biochemistry and Biophysics·Céline Loussert Fonta, Bruno M Humbel
Jan 21, 2015·Archives of Biochemistry and Biophysics·Myan DoCarolyn A Larabell
Jul 15, 2016·Optics Express·Michael MetzgerMarc Brecht
Jun 29, 2016·Scientific Reports·Markko MyllysMaija Vihinen-Ranta
Jan 11, 2017·Chemical Reviews·Toru KondoGabriela S Schlau-Cohen
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Raffaele FaoroThomas P Burg
May 29, 2015·Nature Methods·Pascal de BoerBen N G Giepmans
Apr 5, 2017·Scientific Reports·Joaquín OtónJosé M Carazo
Dec 24, 2019·Journal of Synchrotron Radiation·Haobo BaiYangchao Tian
Oct 19, 2017·Scientific Reports·Emelie FogelqvistHans M Hertz
Jun 18, 2017·Scientific Reports·Vesa AhoMaija Vihinen-Ranta
Sep 10, 2019·Current Opinion in Structural Biology·Jessica Guo, Carolyn A Larabell
Apr 20, 2018·Emerging Topics in Life Sciences·Maria HarkiolakiElizabeth Duke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Journal of Cellular Biochemistry
Bertrand P CinquinCarolyn A Larabell
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Gerry McDermottCarolyn A Larabell
© 2021 Meta ULC. All rights reserved