Correlative gene expression and DNA methylation profiling in lung development nominate new biomarkers in lung cancer

The International Journal of Biochemistry & Cell Biology
Rene CorteseFlorian Eckhardt

Abstract

Although transcriptional control is key for proper lung development, little is known about the possible accompanying epigenetic modifications. Here, we have used gene expression profiling to identify 99 genes that are upregulated in fetal lung and 354 genes that are upregulated in adult lung. From the differentially expressed genes, we analyzed the accompanying 5'-UTR methylation profiles of 43 genes. Out of these, nine genes (COL11A1, MEOX2, SERPINE2, SOX9, FBN2, MDK, COL1A1, LAPTM5 and MARCO) displayed an inverse correlation of their 5'-UTR methylation and the cognate gene expression, suggesting that these genes are at least partially regulated by DNA methylation. Using the differential gene expression/DNA methylation profiles as a guidepost, we identified four genes (MEOX2, MDK, LAPTM5, FGFR3) aberrantly methylated in lung cancer. MEOX2 was uniformly higher methylated in all lung cancer samples (n=15), while the methylation of the other three genes was correlated with either the differentiation state of the tumor (MDK, LAPTM5) or the tumor type itself (FGFR3).

Citations

Oct 23, 2012·Molecular Biology Reports·Ewa BrzeziańskaAdam Antczak
Jun 1, 2011·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Shan Gao, Peter A Andreasen
Jan 13, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Joo Mi YiNita Ahuja
Apr 22, 2009·The Journal of Clinical Investigation·Jing QingAvi Ashkenazi
May 29, 2012·Journal of Cancer Research and Clinical Oncology·Ki-Yeol Kim, In-Ho Cha
Oct 26, 2010·Trends in Endocrinology and Metabolism : TEM·Tommy SeabornYves Tremblay
Feb 11, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Louis RagoliaArzu Büyük
Dec 17, 2008·Pediatric Pulmonology·Steve AbmanUNKNOWN NHLBI working group report
Nov 12, 2014·American Journal of Respiratory Cell and Molecular Biology·Alain CunaNamasivayam Ambalavanan
Feb 10, 2018·Biomarkers in Medicine·Yanjiao RenFengfeng Zhou
Sep 23, 2020·BMC Medical Genomics·Ali Foroughi PourGrzegorz A Rempała
Nov 24, 2017·Journal of Neuro-oncology·Wen WangJizong Zhao
Jan 19, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elisa FrullantiTommaso A Dragani
Feb 2, 2021·International Journal of Genomics·Xiunan LiGuangzhen Wu
Oct 25, 2008·Molecular Cancer·Paul P AnglimIte A Laird-Offringa

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