Correlative Gene Expression to Protective Seroconversion in Rift Valley Fever Vaccinates

PloS One
Richard C LaughlinL Garry Adams

Abstract

Rift Valley fever Virus (RVFV), a negative-stranded RNA virus, is the etiological agent of the vector-borne zoonotic disease, Rift Valley fever (RVF). In both humans and livestock, protective immunity can be achieved through vaccination. Earlier and more recent vaccine trials in cattle and sheep demonstrated a strong neutralizing antibody and total IgG response induced by the RVF vaccine, authentic recombinant MP-12 (arMP-12). From previous work, protective immunity in sheep and cattle vaccinates normally occurs from 7 to 21 days after inoculation with arMP-12. While the serology and protective response induced by arMP-12 has been studied, little attention has been paid to the underlying molecular and genetic events occurring prior to the serologic immune response. To address this, we isolated RNA from whole blood of vaccinated calves over a time course of 21 days before and after vaccination with arMP-12. The time course RNAs were sequenced by RNASeq and bioinformatically analyzed. Our results revealed time-dependent activation or repression of numerous gene ontologies and pathways related to the vaccine induced immune response and its regulation. Additional bioinformatic analyses identified a correlative relationship between ...Continue Reading

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Datasets Mentioned

BETA
GSE71417

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
RNASeq
Assay
electrophoresis
RNA-Seq

Software Mentioned

MATLAB
R
REACTOME
DGBBA
Trimmomatic
DIP
MINT
R package “ GAGE ”
RNASeq
TRANSFAC

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