Cortical bone loss due to skeletal unloading in aldehyde dehydrogenase 2 gene knockout mice is associated with decreased PTH receptor expression in osteocytes

Bone
Takafumi TajimaAkinori Sakai

Abstract

Aldehyde dehydrogenase 2 (ALDH2) is the enzyme that degrades and detoxifies the acetaldehyde produced by alcohol metabolism. In our previous study, we found that compared with wild-type mice (WT), climbing exercises did not increase trabecular bone mass in Aldh2 knockout mice (KO). The purpose of this study was to clarify the effect of the Aldh2 gene on cortical bone structure and on the dynamics of skeletal unloading. Eight-week-old male KO and WT were divided into ground control (GC) or tail suspension (TS) groups for one week (i.e., the KOGC, KOTS, WTGC and WTTS groups). We measured the bone mineral density (BMD) of the femur using dual-energy X-ray absorptiometry. We assessed the femoral morphometry using peripheral quantitative computed tomography (pQCT) and evaluated the femoral cortex histomorphometry, and cortical mRNA using quantitative RT-PCR and cortical bone immunohistostaining. No significant differences were found between the femoral BMD of WTGC and that of WTTS, but the BMD in KOTS was significantly lower than that of KOGC. The pQCT results revealed that the cortical BMD of the femoral diaphysis in KOTS was significantly lower than that of KOGC. Furthermore, the cortical bone area and cortical thickness were sign...Continue Reading

Citations

Feb 13, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yuka WakabayashiKoichi Nakazato
Jan 4, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yasir ArfatCharles H Hocart
Apr 17, 2020·Frontiers in Physiology·Dezhi ZhaoHuiyun Xu
Jan 3, 2021·Calcified Tissue International·Mikkel Bo BrentJesper Skovhus Thomsen
May 20, 2021·Calcified Tissue International·Takuya IshimotoTakayoshi Nakano
Jul 6, 2021·Biochemical Pharmacology·Tianhong ChenJingfeng Li

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