Cortical granule exocytosis in hamster eggs requires microfilaments

Molecular Reproduction and Development
A J DiMaggioJ Stewart-Savage

Abstract

Earlier work has demonstrated that hamster eggs that do not release a second polar body after fertilization in vitro lack a block to polyspermy (Stewart-Savage and Bavister, 1987: Gamete Res 18:333-338). Since polar body release requires microfilaments, the involvement of microfilaments in cortical granule exocytosis was examined. When hamster eggs were treated with cytochalsin B (CB) for 1 hr and then coincubated with sperm for 90 min, there was a dose-dependent increase in both the percentage of eggs with more than one sperm penetrating the zona pellucida and the mean number of sperm that penetrated the zona, with a maximum effect at 20 micrograms CB/ml (100% polypenetration, 3.0 +/- 0.3 sperm/egg). Cytochalasin-treated eggs retained 85% of their cortical granules 55 min after insemination, as compared to unfertilized eggs. Longer time periods did not result in any further reduction. As seen with the scanning confocal microscope, an extensive microfilament network was present in the cortex of untreated eggs, with the cortical granules located within the cortical network. The cortical microfilament network was highly reduced in CB-treated eggs. When viewed with the electron microscope, the same number of cortical granules were...Continue Reading

References

Feb 1, 1992·Biology of Reproduction·P M Wassarman
Dec 1, 1991·Molecular Reproduction and Development·J Stewart-Savage, B D Bavister
Oct 1, 1990·Physiological Reviews·A Carruthers
Nov 1, 1990·Developmental Biology·S D Webster, R W McGaughey
Apr 1, 1988·The Journal of Experimental Zoology·G N CherrD F Katz
Sep 1, 1988·Gamete Research·J Stewart-Savage, B D Bavister
Jan 1, 1986·The Journal of Experimental Zoology·D E Battaglia, P Gaddum-Rosse
Mar 1, 1985·Journal of Reproduction and Fertility·A Niemierko
Aug 1, 1995·Molecular Reproduction and Development·Y TeradaA Yajima
Jan 1, 1993·Cell·R B Kelly
Jul 1, 1993·Biology of Reproduction·L M Legendre, J Stewart-Savage

❮ Previous
Next ❯

Citations

Oct 15, 1998·Molecular Reproduction and Development·T RazR Shalgi
Sep 13, 2001·Molecular Reproduction and Development·J L TremoledaM M Bevers
Apr 30, 2011·ACS Chemical Biology·Alison M KimThomas V O'Halloran
Mar 30, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·A Talmor-CohenR Shalgi
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·E EliyahuR Shalgi
Nov 20, 2009·Anatomia, histologia, embryologia·E GumusC Kaloglu
Jan 18, 2011·Cellular Reprogramming·Chunling BaiGuang-Peng Li
Aug 9, 2006·Reproductive Medicine and Biology·Hiroyuki Suzuki, Yosuke Saito
Jun 26, 1998·Journal of Clinical Laser Medicine & Surgery

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.