Corticoids protect oligodentrocyte precursor cells against cytokine-induced damage

Zentralblatt für Gynäkologie
B FeldhausB Berger

Abstract

In preterm fetuses an ascending intrauterine inflammation before or during birth can be associated with periventricular leukomalacia (PVL). PVL is characterised by a disrupted. myelination [corrected] due to a loss of oligodendrocyte progenitors. Inflammatory cytokines not only induce apoptotic cell death but in addition prevent the differentiation of the immature A2B5 oligodendrocytc progenitors into the myelinating phenotype. The aim of the present study was to clarify whether corticosteroids can protect oligodendrocyte progenitors from such injury. Cultures [corrected] of more than 90 % A2B5 positive progenitors were prepared from neonatal sprague dawley rats. After 1 day in proliferation medium, cells were treated with interferon-gamma [corrected] (IFN-gamma; 10 U/ml) and tumour necrosis factor-alpha [corrected] (TNF-alpha; 10 ng/ml) for 48 h. In addition, corticosterone (C), deoxycorticosterone (DC), or dexamethasone (DEX) (1 micro M) were applied to the incubation medium of the study groups. After treatment cultures were transferred to media containing factors to promote differentiation of progenitors into thc myelinating phenotype. At culture day 9 total cell [corrected] number was determined. In addition, cells were ana...Continue Reading

Citations

Jan 24, 2006·Journal of Neuroscience Research·Divya M ChariRobin J M Franklin
Mar 7, 2021·Biomolecules·Jocelyn M BretonDaniela Kaufer

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