PMID: 8967434May 1, 1996Paper

Corticosteroid receptor mRNA expression is unaffected by corticosteroids in rat kidney, heart, and colon

The American Journal of Physiology
B EscoubetN Farman

Abstract

Hormones can regulate the expression of their own receptor. We have examined whether adrenalectomy (ADX) and hormone replacement by physiological doses of aldosterone or dexamethasone could modulate the expression of glucocorticoid receptor (GR) or mineralocorticoid receptor (MR) at the mRNA level in the rat kidney, distal colon, and heart. Adult rats were adrenalectomized and received or did not receive an infusion of aldosterone (5 micrograms.100 g-1.day-1) or dexamethasone (10 micrograms.100 g-1.day-1). No significant change in steady-state levels of both MR and GR mRNA was detectable by using ribonuclease (RNase) protection assay (RPA) after either ADX or hormone replacement. Because the kidney is heterogeneous with regard to MR expression, RPA was adapted for measurements on microdissected nephron segments. GR mRNA is expressed at comparable levels all along the nephron, whereas MR mRNA is restricted to the distal nephron. No effect of ADX or GR and MR mRNA levels was detected in any nephron segment that was either aldosterone sensitive or insensitive. In situ hybridization confirmed the absence of corticosteroid-dependent modulation of MR mRNA in all kidney cell types. We conclude that variations of corticosteroid status ...Continue Reading

References

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Citations

Mar 1, 1997·Acta Oto-laryngologica·C T TanP Herman
Jan 23, 1999·Current Opinion in Nephrology and Hypertension·N Farman
Nov 6, 2003·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Alexis Bozorg GrayeliEvelyne Ferrary
Sep 7, 2006·American Journal of Physiology. Renal Physiology·Anne M KarinchWiley W Souba
Jul 5, 2003·American Journal of Physiology. Renal Physiology·Rikke NørregaardBoye L Jensen
May 10, 2011·Pflügers Archiv : European journal of physiology·Monica CarmosinoGiovanna Valenti
Nov 5, 1997·American Journal of Respiratory Cell and Molecular Biology·C PlanèsC Clerici
Feb 24, 2001·American Journal of Physiology. Renal Physiology·N Farman, M E Rafestin-Oblin
Jan 31, 1998·The Journal of Biological Chemistry·S DjelidiM Blot-Chabaud
Dec 3, 1999·Biochemical and Biophysical Research Communications·B EscoubetC Clerici
Apr 12, 2000·Kidney International·D Le MenuetM Lombès
May 12, 2004·Molecular and Cellular Endocrinology·Aditi BhargavaDavid Pearce
Jan 4, 1998·Kidney International·D LaouariC Kleinknecht

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