Corticosteroids induce the differential expression of TGFbeta isoforms, receptors and signaling in the gastric mucosa of suckling rats

Regulatory Peptides
Daniela OgiasP Gama

Abstract

Glucocorticoids inhibit the cell proliferation in the gastric epithelium, and induce differentiation, migration and death. The mechanism by which these effects are triggered and controlled is still discussed and can involve the transcription and activation of transforming growth factor beta (TGFbeta). The present study was conducted to evaluate the effect of hydrocortisone short-term treatment on tissue level and distribution of TGFbeta isoforms, receptors and signaling through Smad2/3. To achieve that, 18-day-old rats were injected with hydrocortisone (50 mg/Kg b.wt.) for 0, 1 and 3 h. The stomachs were collected and processed for immunohistochemistry and western blotting. We observed that the treatment for 3 h increased the number of labeled epithelial cells for TGFbeta1 (p < 0.05), decreased the distribution of TGFbeta2 (p < 0.05) and did not alter TGFbeta3, TbetaRI and TbetaRII status. The levels of TGFbeta1 and receptors were checked by western blotting and results corroborate the immunodetection. We also found that phosphorylation of Smad2/3 into Smad2P increased after 3 h (p < 0.05), indicating that the high level TGFbeta1 was active on the cells. We suggest that glucocorticoids differentially regulate the expression of ...Continue Reading

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Citations

Nov 28, 2013·Pathophysiology : the Official Journal of the International Society for Pathophysiology·L P ChurilovL R Mikhailova
Jan 22, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer J P CollinsBoris W Kramer
Jan 25, 2012·Digestive Diseases and Sciences·Seyyed Ali MardMaryam Maleki
Dec 11, 2007·Regulatory Peptides·Eunice Ribeiro de Andrade SáPatrícia Gama
Oct 17, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Daniela OgiasPatrícia Gama

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