Corticotropin-releasing factor (CRF) agonists stimulate testosterone production in mouse leydig cells through CRF receptor-1

Endocrinology
Nadja HeinrichHartmut Berger

Abstract

The influence of CRF on testosterone production in primary mouse Leydig cell cultures was studied, and the type of CRF receptor (CRF-R) involved in this activity was determined. CRF directly stimulated testosterone production in mouse Leydig cells, but did not influence the maximum human (h)CG-induced testosterone production. The effect was time- and dose-dependent, saturable with an EC50 of 2.84 nM for hCRF, antagonized by the CRF antagonist alpha-helical CRF9-41, and accompanied by intracellular cAMP elevation. The rank order of potency of the natural CRF agonists, hCRF, ovine CRF, sauvagine, and urotensin, corresponded to that of their activities on CRF-R1 in rat pituitary cells and also to that reported for this receptor, but not for CRF-R2, when transfected into various cell lines. Furthermore, the difference in response of mouse Leydig cells to [11-D-Thr,12-D-Phe]- and [13-D-His,14-D-Leu]-ovine CRF corresponded to that measured when COS cells expressing CRF-R1 were activated, but was considerably smaller than that observed for activation of COS cells expressing CRF-R2alpha or -R2beta. The messenger RNA encoding the mouse CRF-R1 was detected by RT-PCR in mouse Leydig cell preparations. In contrast to mouse Leydig cells, CR...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1991·Peptides·J H LiveseyR A Donald
Aug 1, 1989·Molecular and Cellular Endocrinology·M G CastroY P Loh
Mar 1, 1989·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·D E GrigoriadisE B De Souza
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Sep 1, 1995·Biology of Reproduction·B M HuangR L Norman
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R ChenW W Vale
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M PerrinW Vale
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T KishimotoM G Rosenfeld
Mar 7, 1995·Regulatory Peptides·M C Olianas, P Onali
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·T W LovenbergT Oltersdorf
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·M SchäferC Stein
Mar 1, 1994·Molecular and Cellular Endocrinology·D GrammatopoulosE W Hillhouse
Aug 1, 1993·The Journal of Clinical Investigation·G MastorakosG P Chrousos
Oct 1, 1993·Journal of Medicinal Chemistry·J F HernandezJ Rivier
May 1, 1996·Endocrinology·C J DonaldsonW W Vale
Apr 1, 1996·Trends in Pharmacological Sciences·D T ChalmersE B De Souza
Jun 1, 1991·Molecular and Cellular Neurosciences·A F SeasholtzS A Camper

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Citations

May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christos C ZouboulisStefan R Bornstein
Aug 23, 2008·Endocrinology·Catherine L Rivier
Jun 1, 2017·International Journal of Molecular Sciences·Mónica Beatriz FrungieriSoledad Paola Rossi
Jan 6, 2006·Journal of Receptor and Signal Transduction Research·Oliver KraetkeMichael Beyermann
Feb 4, 2005·Birth Defects Research. Part A, Clinical and Molecular Teratology·Diana M JuriloffDiana G Mah
May 19, 2000·Annals of the New York Academy of Sciences·M H Perrin, W W Vale
Feb 22, 2000·The Journal of Biological Chemistry·M BeyermannM Bienert

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