Corticotropin releasing factor enhances survival of cultured GABAergic cerebellar neurons after exposure to a neurotoxin

Brain Research. Developmental Brain Research
Paul MadtesRichard W Burry

Abstract

Corticotropin-releasing factor (CRF), in addition to its role as a hormone in the stress response, functions as a neuromodulator in the cerebellum, where it enhances both the spontaneous and amino acid induced firing rate of Purkinje cells. In the cerebellum, CRF and its two types of receptors (CRF-R(1) and CRF-R(2)) are present during cerebellar development at ages that precede the onset of afferent ingrowth and synaptogenesis, suggesting a distinct role during early cerebellar development. The present study was undertaken to determine whether CRF enhances the survival of cerebellar neurons, in particular GABAergic neurons. Primary cultures of cerebellar neurons obtained from embryonic day 18 mice were composed primarily, but not exclusively, of GABAergic neurons. Although CRF-R(1) is present in most neurons in this culture system, when CRF was added to the medium, no significant change in neuronal survival was observed when compared to control cultures. It is possible that a role for CRF is not seen in growth-promoting culture medium at the plating density chosen for this study and may only be evident when the cells have been exposed to conditions that reduce the likelihood of survival, such as exposure to neurotoxins such as...Continue Reading

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Apr 16, 2009·General and Comparative Endocrinology·Sarah L Alderman, Nicholas J Bernier
Jul 28, 2005·Ageing Research Reviews·Nadhim Bayatti, Christian Behl
Jan 11, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jianguo ZhuangFadi Xu
Jan 29, 2020·The Journal of Experimental Biology·Tegan A Williams, Nicholas J Bernier
May 29, 2018·General and Comparative Endocrinology·Sarah L AldermanNicholas J Bernier

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