Corticotropin-releasing hormone stimulates SGK-1 kinase expression in cultured hippocampal neurons via CRH-R1

American Journal of Physiology. Endocrinology and Metabolism
Hui ShengXin Ni

Abstract

Corticotropin-releasing hormone (CRH) has been shown to exhibit various functions in hippocampus. In the present study, we examined the effect of CRH on the expression of serum/glucocorticoid-inducible protein kinase-1 (SGK-1), a novel protein kinase, in primary cultured hippocampal neurons. A dose-dependent increase in mRNA and protein levels of SGK-1 as well as frequency of SGK-1-positive neurons occurred upon exposure to CRH (1 pmol/l to 10 nmol/l). These effects can be reversed by the specific CRH-R1 antagonist antalarmin but not by the CRH-R2 antagonist astressin 2B. Blocking adenylate cyclase (AC) activity with SQ22536 and PKA with H89 completely prevented CRH-induced mRNA and protein expression of SGK-1. Blockage of PLC or PKC did not block CRH-induced SGK-1 expression. Our results suggest that CRH act on CRH-R1 to stimulate SGK-1 mRNA and protein expression in cultured hippocampal neurons via a mechanism that is involved in AC/PKA signaling pathways.

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Citations

Jun 10, 2010·The Journal of Physiology·Florian LangUndine E Lang
Nov 30, 2011·Neuropharmacology·Richard L HaugerRobert H Oakley
Aug 15, 2015·Neuroscience and Biobehavioral Reviews·R Parrish WatersCliff H Summers
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Sep 19, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Reggie Hui-Chao LeeHung Wen Lin
Apr 25, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity

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