Cortisol and TGF-beta inhibit secretion of platelet-activating factor-acetylhydrolase in a monocyte-macrophage model system [corrected

Molecular Human Reproduction
K K OkumuraT Mori

Abstract

Recent studies have suggested that platelet activating factor (PAF) plays an important role in various reproductive functions, including ovulation, implantation and parturition, and that the local concentration of PAF is modulated by PAF-acetylhydrolase (PAF-AH), a potent PAF inactivator. In this study, we investigated the possible effects of various bioactive substances, which are present at high concentrations in the human pregnant uterus, on PAF-AH secretion from decidual macrophages using a monocyte-macrophage model system, human myelocytic leukaemia cells (HL-60). By treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA), HL-60 cells were transformed to macrophage-like cells, which secreted PAF-AH into the culture medium time- and dose-dependently. After treatment with 10(-8) M TPA, the effects of various substances on the secretion of PAF-AH were examined. Among the substances examined, cortisol and TGF-beta suppressed PAF-AH secretion from TPA-stimulated HL-60 cells in a significant and dose-dependent way. Endothelin, epidermal growth factor, and brain natriuretic peptide had no significant effect on PAF-AH secretion from TPA-stimulated HL-60 cells. These results suggest that local PAF concentrations in the pregnant u...Continue Reading

Citations

Feb 5, 2000·Journal of Reproductive Immunology·S N HudsonS A Robertson
Jun 18, 1999·Journal of Medical Primatology·E DiazW E Roudebush
Feb 15, 2007·Reproductive Biomedicine Online·Atef AliPierre Miron
Feb 10, 2012·The Journal of Surgical Research·Amy T MakleyTimothy A Pritts
Nov 18, 2000·Biochimica Et Biophysica Acta·L W Tjoelker, D M Stafforini

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