Cortisol increases transfection efficiency of cells

FEBS Letters
A G BernasconiI Galli

Abstract

DNA uptake can be facilitated by addition of physiological amounts of 11beta-hydroxy glucocorticosteroids (such as cortisol) during transfection. In the presence of cortisol, but not of the inactive 11-keto glucocorticoid cortisone, twice as many cells uptake and express the reporter gene. The effect is specific and dose-dependent; the amounts of glucocorticosteroids needed to enhance transfection efficiency are in the nanomolar range, which corresponds to the dissociation constant of glucocorticoids for the glucocorticoid receptor in vitro. This effect can be abolished by an excess of the glucocorticoid antagonist RU486. We infer that the activated cytoplasmic glucocorticoid receptors enhance nuclear translocation of the incoming transfected DNA.

References

Apr 15, 1975·International Journal of Cancer. Journal International Du Cancer·G KimuraJ Summers
May 1, 1989·Journal of Steroid Biochemistry·M BeatoE P Slater
Oct 1, 1987·Endocrinology·W Sutanto, E R De Kloet
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F PayvarK R Yamamoto
Jan 1, 1984·Journal of Steroid Biochemistry·J A GustafssonK Yamamoto
Aug 1, 1984·Journal of Steroid Biochemistry·P L BallardI Gross
May 1, 1982·Steroids·J R SchreiberG F Erickson
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·A P WrightJ A Gustafsson
Jan 1, 1997·The Biochemical Journal·K J Fisher, J M Wilson

❮ Previous
Next ❯

Citations

Oct 20, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abby M KellyAngela K Pannier
Apr 12, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rajesh R NairLindsay A Schwarz
Aug 29, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Victor Shahin
Aug 20, 2005·Journal of Cellular Physiology·Lilian KastrupVictor Shahin
Nov 20, 2018·Biotechnology and Bioengineering·Andrew HamannAngela K Pannier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.