Corto and DSP1 interact and bind to a maintenance element of the Scr Hox gene: understanding the role of Enhancers of trithorax and Polycomb.

BMC Biology
Juliette SalvaingFrédérique Peronnet

Abstract

Polycomb-group genes (PcG) encode proteins that maintain homeotic (Hox) gene repression throughout development. Conversely, trithorax-group (trxG) genes encode positive factors required for maintenance of long term Hox gene activation. Both kinds of factors bind chromatin regions called maintenance elements (ME). Our previous work has shown that corto, which codes for a chromodomain protein, and dsp1, which codes for an HMGB protein, belong to a class of genes called the Enhancers of trithorax and Polycomb (ETP) that interact with both PcG and trxG. Moreover, dsp1 interacts with the Hox gene Scr, the DSP1 protein is present on a Scr ME in S2 cells but not in embryos. To understand better the role of ETP, we addressed genetic and molecular interactions between corto and dsp1. We show that Corto and DSP1 proteins co-localize at 91 sites on polytene chromosomes and co-immunoprecipitate in embryos. They interact directly through the DSP1 HMG-boxes and the amino-part of Corto, which contains a chromodomain. In order to search for a common target, we performed a genetic interaction analysis. We observed that corto mutants suppressed dsp11 sex comb phenotypes and enhanced AntpScx phenotypes, suggesting that corto and dsp1 are simultan...Continue Reading

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Citations

May 23, 2013·Omics : a Journal of Integrative Biology·Shahram GolbabapourA Hamid A Hadi
Mar 3, 2010·Genesis : the Journal of Genetics and Development·O LamiableM Decoville
May 23, 2015·Epigenetics & Chromatin·Camille A DupontFrédérique Peronnet
Feb 21, 2008·PloS One·Juliette SalvaingFrédérique Peronnet
Dec 23, 2006·Development·Leonie Ringrose, Renato Paro

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Methods Mentioned

BETA
immunoprecipitation
co-immunoprecipitation
pull-down
PCR

Software Mentioned

ImageJ

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