PMID: 11313140Apr 21, 2001Paper

Corynebacterium diphtheriae surface proteins as adhesins to human erythrocytes

FEMS Microbiology Letters
A V ColomboAna L Mattos-Guaraldi

Abstract

Corynebacterium diphtheriae strains express non-fimbrial surface proteins able to recognize and bind to specific host cells receptors. Protein extracts were obtained from bacterial cells by mechanical process and ammonium sulfate precipitation at 25 and 45% (w/v) saturation. SDS-PAGE analysis of the extracts detected two polypeptide bands of 67 and 72 kDa, named 67-72 p. The 67-72 p, rabbit anti-67-72 p IgG antibodies as well as human gastric mucin, N-acetylneuraminic acid and N-acetyl D-glucosamine molecules were able to inhibit bacterial hemagglutination. Hemagglutination assays using 67-72 p-coated latex beads and Western blot analysis of biotin-labeled 67-72 p and erythrocyte receptors demonstrated the binding of 67-72 p to human erythrocyte membranes. Immunolabeled colloidal gold-A protein transmission electron microscopy using anti-67-72 p revealed a diffuse distribution of non-fimbrial 67-72 p on the surface of C. diphtheriae strains of both sucrose-fermenting and non-fermenting biotypes. Non-fimbrial lectin-like surface 67-72 p may play a role as adhesins in bacterial attachment thereby facilitating the early steps in pathogenesis of both toxigenic and non-toxigenic C. diphtheriae.

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Jan 1, 1994·Methods in Enzymology·P FalkS Normark
Jan 1, 1997·International Review of Cytology·S Kelm, R Schauer
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Jan 27, 1999·FEMS Microbiology Letters·A L Mattos-GuaraldiA F Andrade
Dec 2, 2000·Microbes and Infection·A L Mattos-GuaraldiG A Pereira

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Citations

Oct 24, 2002·FEMS Microbiology Letters·Raphael HirataAna Luíza Mattos-Guaraldi
Oct 9, 2003·Applied and Environmental Microbiology·Lílian de Oliveira MoreiraAna Luiza Mattos-Guaraldi
Jun 1, 2013·ISRN Microbiology·Andreas Burkovski
Jan 31, 2015·Microbiology·Camila Azevedo AntunesAndreas Burkovski
Dec 3, 2016·Journal of Medical Microbiology·Renata Stavracakis PeixotoAna Luíza Mattos-Guaraldi
Feb 9, 2018·AIMS Microbiology·Lisa Ott
Mar 31, 2004·Memórias do Instituto Oswaldo Cruz·Ana Luíza Mattos-GuaraldiRaphael Hirata Júnior
May 17, 2012·European Biophysics Journal : EBJ·Johannes RheinlaenderTilman E Schäffer
Nov 30, 2011·Revista de saúde pública·Alexandre Alves de Souza de Oliveira DiasAna Luíza Mattos Guaraldi
Aug 23, 2016·Future Microbiology·Elena HackerAndreas Tauch
Sep 8, 2004·Microbial Pathogenesis·Lucia BertucciniChristina von Hunolstein

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