Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids

Microbial Cell Factories
Nicolai Kallscheuer, Jan Marienhagen

Abstract

Hydroxybenzoic acids are industrially relevant aromatic compounds, which also play key roles in the microbial carbon metabolism, e.g., as precursors for the synthesis of cofactors or metal-chelating molecules. Due to its pronounced resistance to aromatics Corynebacterium glutamicum represents an interesting platform for production of these compounds. Unfortunately, a complex catabolic network for aromatic molecules prevents application of C. glutamicum for microbial production of aromatic compounds other than aromatic amino acids, which cannot be metabolized by this microorganism. We completed the construction of the platform strain C. glutamicum DelAro5, in which the deletion of altogether 27 genes in five gene clusters abolished most of the peripheral and central catabolic pathways for aromatic compounds known in this microorganism. The obtained strain was subsequently applied for the production of 2-hydroxybenzoate (salicylate), 3-hydroxybenzoate, 4-hydroxybenzoate and protocatechuate, which all derive from intermediates of the aromatic amino acid-forming shikimate pathway. For an optimal connection of the designed hydroxybenzoate production pathways to the host metabolism, C. glutamicum was additionally engineered towards i...Continue Reading

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Citations

Nov 6, 2018·Journal of Industrial Microbiology & Biotechnology·Jillian M HagelPeter J Facchini
Feb 2, 2019·Organic & Biomolecular Chemistry·Mads J GrüningerJennifer N Andexer
Jun 11, 2020·Biomolecules·Egle ValancieneNaglis Malys
Jun 28, 2019·Frontiers in Bioengineering and Biotechnology·Christoph LenzenNick Wierckx
Feb 28, 2019·Microbial Cell Factories·Damla HuccetogullariSang Yup Lee
Nov 7, 2019·Applied Microbiology and Biotechnology·Nicolai KallscheuerJan Marienhagen
Apr 2, 2019·Engineering in Life Sciences·Yiyao ZhouHaoran Zhang
Oct 18, 2019·Frontiers in Bioengineering and Biotechnology·Kareen H VeldmannVolker F Wendisch
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Songwei WangXuehong Zhang
Feb 3, 2021·Chembiochem : a European Journal of Chemical Biology·Douglas WeberDörte Rother
Jun 12, 2020·Microorganisms·Tatjana WalterVolker F Wendisch
Aug 10, 2021·ACS Synthetic Biology·Lion Konstantin FlachbartJan Marienhagen
Aug 11, 2021·Journal of Microbiology and Biotechnology·Eunhwi ParkEung-Soo Kim

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Methods Mentioned

BETA
PCR
phosphotransferase
genetic modification
genetic modifications

Software Mentioned

Electra Cloning

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