Corynebacterium glutamicum exhibits a membrane-related response to a small ferrocene-conjugated antimicrobial peptide.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Benjamin FränzelDirk Wolters

Abstract

Multiresistant bacteria are becoming more and more widespread. It is therefore necessary to have new compound groups in hand, such as small cationic peptides, to cope with these challenges. In this work, we present a comprehensive approach by monitoring protein expression profiles in a gram-positive bacterium (Corynebacterium glutamicum) to investigate the cellular response to such a compound, a ferrocene-conjugated arginine- and tryptophan-rich pentapeptide. To achieve this, a proteomic outline was performed where the compound-treated sample was compared with an untreated control. This study comprises more than 900 protein identifications, including numerous integral membrane proteins, and among these 185 differential expressions. Surprisingly, unregulated catalase and no elevated H(2)O(2) levels demonstrate that no oxidative stress occurs after treatment with the iron-containing compound as a consequence of the potential Fenton reaction. A sufficient iron supply is evidenced by the iron-containing protein aconitase and SufB (the latter belongs to an iron-sulfur cluster assembly system) and decreased levels of ATP-binding-cassette-type cobalamin/Fe(3+) siderophore transporters. The organometallic peptide antibiotic targets the...Continue Reading

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Citations

Dec 5, 2012·Beilstein Journal of Organic Chemistry·H Bauke AlbadaNils Metzler-Nolte
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michaela WenzelJulia Elisabeth Bandow
Nov 26, 2015·Biochimica Et Biophysica Acta·Michaela WenzelJulia E Bandow
Jul 5, 2011·Proteomics·Michaela Wenzel, Julia E Bandow
Jun 16, 2011·Proteomics·Ansgar PoetschAndreas Burkovski
Nov 19, 2015·Microbial Cell Factories·Xiaoli YuQingsheng Qi
May 18, 2016·International Journal of Molecular Sciences·Andre Reinhardt, Ines Neundorf
Sep 15, 2016·Chemical Reviews·Bauke Albada, Nils Metzler-Nolte
Aug 10, 2012·Current Protocols in Microbiology·Nadja Raatschen, Julia Elisabeth Bandow
Mar 15, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Jan HoyerInes Neundorf
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Ann-Britt Schäfer, Michaela Wenzel
Sep 28, 2017·Accounts of Chemical Research·Bauke Albada, Nils Metzler-Nolte

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