Corynebacterium ulcerans 0102 carries the gene encoding diphtheria toxin on a prophage different from the C. diphtheriae NCTC 13129 prophage.

BMC Microbiology
Tsuyoshi SekizukaMasaaki Iwaki

Abstract

Corynebacterium ulcerans can cause a diphtheria-like illness, especially when the bacterium is lysogenized with a tox gene-carrying bacteriophage that produces diphtheria toxin. Acquisition of toxigenicity upon phage lysogenization is a common feature of C. ulcerans and C. diphtheriae. However, because of a lack of C. ulcerans genome information, a detailed comparison of prophages has not been possible between these two clinically important and closely related bacterial species. We determined the whole genome sequence of the toxigenic C. ulcerans 0102 isolated in Japan. The genomic sequence showed a striking similarity with that of Corynebacterium pseudotuberculosis and, to a lesser extent, with that of C. diphtheriae. The 0102 genome contained three distinct prophages. One of these, ΦCULC0102-I, was a tox-positive prophage containing genes in the same structural order as for tox-positive C. diphtheriae prophages. However, the primary structures of the individual genes involved in the phage machinery showed little homology between the two counterparts. Taken together, these results suggest that the tox-positive prophage in this strain of C. ulcerans has a distinct origin from that of C. diphtheriae NCTC 13129.

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Citations

Jun 25, 2014·Emerging Infectious Diseases·Vartul SangalPaul A Hoskisson
Jan 11, 2014·Genome Announcements·Andréia do Socorro de Sousa SilvaRommel Thiago Jucá Ramos
Feb 9, 2016·Microbiology and Immunology·Chihiro KatsukawaMasaaki Iwaki
Mar 15, 2015·Genome Announcements·Leandro de Jesus BenevidesLuis Carlos Guimarães
Jan 28, 2015·Emerging Infectious Diseases·Dominik M MeinelAndreas Sing
Aug 23, 2016·Future Microbiology·Elena HackerAndreas Tauch
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Oct 14, 2017·JMM Case Reports·Ken OtsujiMitsumasa Saito
Sep 13, 2019·Journal of Clinical Medicine·Cátia PacíficoFriederike Hilbert
Dec 6, 2019·Nature Reviews. Disease Primers·Naresh Chand SharmaThandavarayan Ramamurthy
Oct 19, 2019·Emerging Infectious Diseases·Ken OtsujiMitsumasa Saito
Sep 19, 2020·Emerging Infectious Diseases·Tsuyoshi SekizukaMasaaki Iwaki
Jun 6, 2020·Antonie van Leeuwenhoek·Jens MöllerVartul Sangal

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ClustalW2
GE
figtree
ACT
pe
Maq
BLASTN
ABySS
Cluster Generation
IMC

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