Abstract
We examined the secretion of immunoreactive (ir) atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in primary cultures of atrial and ventricular cardiocytes from neonatal rats, and also investigated the stimulatory effect of endothelin-1 (ET-1) on ir-ANP and ir-BNP release from these cells. After 2 days of culture, rat atrial and ventricular cardiocytes secreted both ir-ANP and ir-BNP into serum-free medium in a time-dependent manner. The amount of ir-BNP released per 10(5) ventricular cells was approximately 60% to 70% (1.8 +/- 0.5 pg/h, 2.5 +/- 0.4 pg/2 h, 2.6 +/- 0.4 pg/4 h) of that released from atrial cells (2.8 +/- 0.4 pg/h, 3.5 +/- 0.6 pg/2 h, 4.6 +/- 0.6 pg/4 h), although ir-ANP was secreted almost entirely from atrial cells. ET-1 clearly stimulated secretion of both ir-ANP and ir-BNP in atrial and ventricular cardiocytes. These observations indicate that ir-BNP is cosecreted with ir-ANP not only from atrial cardiocytes, but also from ventricular cardiocytes in the rat, and that ET-1 stimulates the secretion of these natriuretic peptides by a direct mechanism, not through a hemodynamic change.
References
Feb 1, 1992·Hypertension·M KohnoT Takeda
Jun 1, 1991·The Journal of Clinical Investigation·M KohnoT Takeda
Mar 31, 1990·Lancet·M MukoyamaH Imura
May 31, 1991·Biochemical and Biophysical Research Communications·M AburayaH Matsuo
Jan 1, 1990·FEBS Letters·Y KambayashiH Imura
Jan 15, 1990·FEBS Letters·T IidaF Marumo
Jun 1, 1990·Metabolism: Clinical and Experimental·M KohnoT Takeda
Dec 14, 1990·Biochemical and Biophysical Research Communications·N YokotaH Matsuo
Apr 1, 1990·Endocrinology·Y OgawaM Jougasaki
Mar 31, 1988·Nature·M YanagisawaT Masaki
Aug 30, 1988·Biochemical and Biophysical Research Communications·Y FukudaT Masaki
Nov 15, 1989·Biochemical and Biophysical Research Communications·Y FukudaY Kobayashi
May 15, 1989·Biochemical and Biophysical Research Communications·Y HirataF Marumo
Aug 30, 1989·Biochemical and Biophysical Research Communications·M AburayaH Matsuo
Dec 31, 1985·Biochemical and Biophysical Research Communications·N KatsubeP Needleman
Jan 14, 1986·Biochemical and Biophysical Research Communications·M KohnoT Takeda
Mar 3, 1988·Nature·T SudohH Matsuo
Sep 15, 1988·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Jul 1, 1980·Circulation Research·S WallensteinJ L Fleiss
Citations
Aug 1, 1996·Endocrine·K L KingJ P Mather
Sep 12, 1996·European Journal of Pharmacology·Y BézieS Laurent
Jan 13, 2004·Journal of Cardiovascular Pharmacology·Yi ZhangJohn D Horowitz
Apr 1, 1999·Annual Review of Physiology·G ThibaultR Garcia
Jan 21, 2011·Physiological Reviews·Donald E KohanDavid M Pollock
Dec 13, 2005·American Heart Journal·Sonia RalliGregg C Fonarow
Apr 5, 2005·Immunopharmacology and Immunotoxicology·Ki-Chan HaSoo-Wan Chae
Sep 25, 2001·The American Journal of Gastroenterology·E FábregaF Pons-Romero
Mar 10, 2001·Biological Research for Nursing·S D Kim, M R Piano
Mar 1, 1994·Circulation Research·G ThibaultE L Schiffrin
Nov 2, 2002·Hypertension·Miriam T RademakerA Mark Richards
Dec 31, 1997·Circulation·J MaggaH Ruskoaho
Nov 14, 1997·Circulation Research·J MaggaH Ruskoaho
Sep 8, 2017·American Journal of Physiology. Renal Physiology·Lynnette R Montgomery, Charles H Hubscher
Oct 6, 2001·Annals of Clinical Biochemistry·R Kelly, A D Struthers