Cosecretion of atrial and brain natriuretic peptides stimulated by endothelin-1 from cultured rat atrial and ventricular cardiocytes

Metabolism: Clinical and Experimental
T HorioT Takeda

Abstract

We examined the secretion of immunoreactive (ir) atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in primary cultures of atrial and ventricular cardiocytes from neonatal rats, and also investigated the stimulatory effect of endothelin-1 (ET-1) on ir-ANP and ir-BNP release from these cells. After 2 days of culture, rat atrial and ventricular cardiocytes secreted both ir-ANP and ir-BNP into serum-free medium in a time-dependent manner. The amount of ir-BNP released per 10(5) ventricular cells was approximately 60% to 70% (1.8 +/- 0.5 pg/h, 2.5 +/- 0.4 pg/2 h, 2.6 +/- 0.4 pg/4 h) of that released from atrial cells (2.8 +/- 0.4 pg/h, 3.5 +/- 0.6 pg/2 h, 4.6 +/- 0.6 pg/4 h), although ir-ANP was secreted almost entirely from atrial cells. ET-1 clearly stimulated secretion of both ir-ANP and ir-BNP in atrial and ventricular cardiocytes. These observations indicate that ir-BNP is cosecreted with ir-ANP not only from atrial cardiocytes, but also from ventricular cardiocytes in the rat, and that ET-1 stimulates the secretion of these natriuretic peptides by a direct mechanism, not through a hemodynamic change.

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Citations

Jan 13, 2004·Journal of Cardiovascular Pharmacology·Yi ZhangJohn D Horowitz
Apr 1, 1999·Annual Review of Physiology·G ThibaultR Garcia
Jan 21, 2011·Physiological Reviews·Donald E KohanDavid M Pollock
Apr 5, 2005·Immunopharmacology and Immunotoxicology·Ki-Chan HaSoo-Wan Chae
Sep 25, 2001·The American Journal of Gastroenterology·E FábregaF Pons-Romero
Mar 10, 2001·Biological Research for Nursing·S D Kim, M R Piano
Nov 2, 2002·Hypertension·Miriam T RademakerA Mark Richards
Sep 8, 2017·American Journal of Physiology. Renal Physiology·Lynnette R Montgomery, Charles H Hubscher
Oct 6, 2001·Annals of Clinical Biochemistry·R Kelly, A D Struthers

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