Apr 19, 2020

Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes

BioRxiv : the Preprint Server for Biology
Julianne R BrownK. Harris

Abstract

We aim to test four one-step RT real-time mastermix options for use in SARS-CoV2 real-time PCR, with three primer/probe assays targeting the N gene. The lower limit of detection is determined using a SARS-CoV2 N gene RNA transcript dilution series (to 1 copy/ul) and verified using 74 nose and throat swabs. The N2 assay demonstrates the most sensitive detection of SARS-Cov2 RNA. Three of the four mastermixes performed well, with the Takara One Step PrimeScript III RT-PCR Kit mastermix demonstrating improved performance at the lower limit of detection.

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