Cost analysis based on bioreactor cultivation conditions: Production of a soluble recombinant protein using Escherichia coli BL21(DE3)

Biotechnology Reports
Valdemir M CardosoTeresa C Zangirolami

Abstract

The impact of cultivation strategy on the cost of recombinant protein production is crucial for defining cost-effective bioreactor operation conditions. This paper presents a methodology to estimate and compare cost impacts related to utilities as well as medium composition, using simple design equations and accessible data. Data from batch bioreactor cultures were used as case study involving the production of pneumococcal surface protein A, a soluble recombinant protein, employing E. coli BL21(DE3). Cultivation strategies and corresponding process costs covered a wide range of operational conditions, including different media, inducers, and temperatures. The core expenses were related to the medium and cooling. When the price of peptone was above the threshold value of US$ 30/kg, defined medium became the best choice. IPTG and temperatures around 32 °C led to shorter cultures and lower PspA4Pro production costs. The procedure offers a simple, accessible theoretical tool to identify cost-effective production strategies using bioreactors.

Citations

Oct 13, 2020·Applied Biochemistry and Biotechnology·Maria de Fátima M de FreitasLuciana R B Gonçalves
Oct 29, 2020·Biotechnology for Biofuels·Katharina NovakStefan Pflügl
Oct 21, 2020·Advanced Drug Delivery Reviews·Laura ChambreDavid L Kaplan
Mar 31, 2021·Annual Review of Chemical and Biomolecular Engineering·Cynthia NiKristala L J Prather
May 30, 2021·Biotechnology Journal·Cynthia NiKristala L J Prather
Jul 28, 2021·Enzyme and Microbial Technology·Victor E Balderas HernándezAntonio De Leon-Rodriguez
Aug 17, 2021·Frontiers in Bioengineering and Biotechnology·Daniel SiebertBastian Blombach

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Methods Mentioned

BETA
circular dichroism

Software Mentioned

SuperSys
Q HEAT
ImageJ
HCDC

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