Cost-effective paper-based electrochemical immunosensor using a label-free assay for sensitive detection of ferritin

The Analyst
Suchanat BoonkaewOrawon Chailapakul

Abstract

Ferritin, a blood cell protein containing iron, is a crucial biomarker that is used to estimate the risk assessment of iron deficiency anemia. For point-of-care analysis, a reliable, cost-effective, selective, sensitive, and portable tool is extremely necessary. In this study, a label-free electrochemical immunosensor for detecting ferritin using a paper-based analytical device (ePAD) was created. The device pattern was custom designed onto filter paper to successfully fabricate a deliverable immunosensor. Graphene oxide was first modified onto the working electrode using an inkjet printing technique. An activation step of the electrode surface was then performed using standard 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-hydroxysulfosuccinimide (sulfo-NHS) chemistry. Anti-ferritin antibodies were covalently immobilized onto the amine-reactive ester surface. The amount of ferritin was monitored by observing the electrochemical signal of the selected redox couple by differential pulse voltammetry (DPV). In the presence of ferritin, the sensor showed a considerable decrease in electrochemical response in a concentration-dependent manner. In contrast, there was no observable change in current response detected in the abse...Continue Reading

References

Oct 14, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hyun C YoonSang Yo Byun
Dec 6, 2005·Biosensors & Bioelectronics·Xiuhua ZhangYao Xiao
Oct 7, 2008·Blood Reviews·Mary Ann KnovichFrank M Torti
Jun 3, 2009·Analytical Chemistry·Wijitar DungchaiCharles S Henry
Jun 23, 2011·Therapeutic Advances in Gastroenterology·Terri D Johnson-Wimbley, David Y Graham
Dec 18, 2013·Biosensors & Bioelectronics·Jie HuFeng Xu
Jul 11, 2014·Metallomics : Integrated Biometal Science·Yao Ren, Thomas Walczyk
Sep 19, 2014·Nanoscale Research Letters·Nan-Fu ChiuKou-Chen Liu
Nov 23, 2017·Journal of Natural Medicines·Seiichi SakamotoSatoshi Morimoto
Feb 7, 2018·Food Chemistry·Chan-Chiung LiuKuan-Lin Chen
Jun 9, 2018·Clinical Case Reports·Esa T Soppi
Nov 24, 2018·Analytical Biochemistry·Hedieh Haji-HashemiMohammad Reza Ganjali
Mar 10, 2019·Biosensors & Bioelectronics·Sabine SzuneritsRabah Boukherroub
May 16, 2019·Analytica Chimica Acta·Natchanon Taprab, Yupaporn Sameenoi

❮ Previous
Next ❯

Citations

Aug 9, 2020·Biosensors·Mayank GargNicole Pamme
Oct 24, 2020·Analytical and Bioanalytical Chemistry·Minu MathewChandra Sekhar Rout
May 20, 2021·ACS Sensors·Juliane R SempionattoJoseph Wang
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Enrica RosatoCristian D'Ovidio
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Jul 3, 2021·Sensors·Reena Sri SelvarajanAzrul Azlan Hamzah

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
infrared spectroscopy
ELISA

Software Mentioned

Adobe Illustrator
ASSURED

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Related Papers

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Watthanachai JumpathongKontad Ounnunkad
Journal of Nanoscience and Nanotechnology
Rahul SaxenaSudha Srivastava
© 2022 Meta ULC. All rights reserved