Cost-effective production of recombinant peptides in Escherichia coli

New Biotechnology
Rosa GaglioneAngela Arciello

Abstract

Among bioactive peptides, cationic antimicrobial peptides (AMPs), also referred to as host defence peptides (HDPs), are valuable tools to treat infections, being able to kill a wide variety of microbes directly and/or modulate host immunity. HDPs have great therapeutic potential against antibiotic-resistant bacteria, viruses and even parasites. However, high manufacturing costs have greatly limited their development as drugs, thus highlighting the need to develop novel and competitive production strategies. Here, a cost-effective procedure was established to produce the high amounts of peptides required for basic and clinical research. Firstly, a novel culture medium was designed, which was found to support significantly higher cell densities and recombinant expression levels of peptides under test compared to conventional media. The procedure has been also efficiently scaled up by using a 5 L fermenter, while the costs have been lowered significantly by developing a successful auto-induction strategy, which has been found to support higher yields of target constructs and cell biomass compared to conventional strategies based on expression induction by IPTG. Interestingly, it was estimated that by increasing production scale fr...Continue Reading

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Citations

Mar 4, 2020·Current Topics in Medicinal Chemistry·Jiarui LiEster Boix
Apr 28, 2020·Current Topics in Medicinal Chemistry·Rosa GaglioneAngela Arciello
Aug 28, 2020·Frontiers in Chemistry·Katia PaneMonica Franzese
Mar 21, 2020·International Journal of Molecular Sciences·Rosa GaglioneAngela Arciello
Jan 17, 2021·Journal of Biomedical Science·Mohd Ishtiaq AnasirChit Laa Poh
Feb 13, 2021·Applied Microbiology and Biotechnology·Eliana Dell'OlmoAngela Arciello
Dec 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Verónica RojasFanny Guzmán
Jun 3, 2021·Pharmaceuticals·Aizhan AbdildinovaYoung-Dae Gong
Jul 2, 2021·Frontiers in Cellular and Infection Microbiology·Antonio MorettaPatrizia Falabella
Aug 14, 2020·Biomacromolecules·Pedro Salas-AmbrosioColin Bonduelle

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