Cost of surviving sepsis: a novel model of recovery from sepsis in Drosophila melanogaster

Intensive Care Medicine Experimental
Ata Murat KaynarDerek C Angus

Abstract

Multiple organ failure, wasting, increased morbidity, and mortality following acute illness complicates the health span of patients surviving sepsis. Persistent inflammation has been implicated, and it is proposed that insulin signaling contributes to persistent inflammatory signaling during the recovery phase after sepsis. However, mechanisms are unknown and suitable pre-clinical models are lacking. We therefore developed a novel Drosophila melanogaster model of sepsis to recapitulate the clinical course of sepsis, explored inflammation over time, and its relation to impaired mobility, metabolic disturbance, and changes in lifespan. We used wild-type (WT), Drosomycin-green fluorescent protein (GFP), and NF-κB-luc reporter male Drosophila melanogaster 4-5 days of age (unmanipulated). We infected Drosophila with Staphylococcus aureus (infected without treatment) or pricked with aseptic needles (sham). Subsets of insects were treated with oral linezolid after the infection (infected with antibiotics). We assessed rapid iterative negative geotaxis (RING) in all the groups as a surrogate for neuromuscular functional outcome up to 96 h following infection. We harvested the flies over the 7-day course to evaluate bacterial burden, in...Continue Reading

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Citations

Jun 21, 2017·International Journal of Molecular Sciences·Edmund J Miller, Helena M Linge
Aug 26, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Juliana Marzari RossatoJoão Batista Teixeira da Rocha
Jun 3, 2021·International Journal of Molecular Sciences·Krzysztof LaudanskiKathleen E Sullivan

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