PMID: 8946677Oct 1, 1996Paper

Costimulation by CD28 sFv expressed on the tumor cell surface or as a soluble bispecific molecule targeted to the L6 carcinoma antigen

Tissue Antigens
M S HaydenJ A Ledbetter

Abstract

Interaction of the CD80 (B7-1) and CD86 (B7-2) molecules on antigen presenting cells with the receptors CD28 and CTLA-4 on T cells generates signals important in the regulation of immune responses. Because this receptor system involves multiple receptor-ligand interactions, determining the function for individual receptors has been difficult. One approach is the use of antibodies and their derivatives with singular specificity as substitute ligands to explore the activities of these molecules. We have constructed recombinant mono- and bi-specific sFv molecules specific for the CD28 receptor that are capable of binding and generating costimulatory signals to activate T cells. We demonstrate that these soluble molecules are capable of higher levels of costimulation than soluble CD80Ig at equivalent concentrations. We also constructed artificial adhesion receptors on the cell surface using two different CD28-specific sFvIgs fused to the CD80 cytoplasmic and transmembrane domains. In this report, we compared costimulation by a soluble bispecific (alpha CD28-alpha L6) single chain sFvIg fusion protein to that generated by L6 antigen positive (L6+) H3347 tumor cells transduced with cell surface expressed forms of alpha CD28 sFv's. We...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S MarkenA Aruffo
Sep 1, 1991·The Journal of Experimental Medicine·P S LinsleyJ A Ledbetter
Jan 24, 1991·Nature·G Winter, C Milstein
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C QueenT A Waldmann
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U D Staerz, M J Bevan
Nov 1, 1986·Somatic Cell and Molecular Genetics·G UrlaubJ Hamlin
Jan 1, 1987·European Journal of Immunology·A Lanzavecchia, D Scheidegger
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G JungH J Müller-Eberhard
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L K GillilandH Waldmann
Aug 1, 1986·Cancer Research·I HellströmK E Hellström
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A LiuH N Eisen
Oct 6, 1983·Nature·C Milstein, A C Cuello
Feb 25, 1981·Nucleic Acids Research·W AltenburgerH G Zachau
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R OlloF Rougeon
Feb 1, 1994·The Journal of Experimental Medicine·L ChenK E Hellström
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·R S McHughP Selvaraj
Nov 1, 1995·The Journal of Experimental Medicine·T C WuD M Pardoll
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M MackP Kufer
Jun 1, 1995·Nature Structural Biology·P D JeffreyS Sheriff
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BaskarL H Glimcher
Aug 1, 1994·Immunity·T L WalunasJ A Bluestone
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L DuanR J Pomerantz
Jun 1, 1993·Immunological Reviews·S W UmlaufR H Schwartz
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W A MarascoS Y Chen
Mar 22, 1996·Science·D R LeachJ P Allison
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·J C BeckerR A Reisfeld

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Citations

Apr 3, 2001·Biotechnology and Bioengineering·K W LiaoS R Roffler
Feb 27, 2001·Journal of Immunological Methods·M D GriffinJ A Bluestone
Apr 1, 1997·Current Opinion in Immunology·M S HaydenJ A Ledbetter
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hye J KimByungsuk Kwon
Jan 9, 2004·Expert Review of Vaccines·Karl Erik Hellstrom, Ingegerd Hellstrom
May 16, 2008·Medicinal Research Reviews·Tian-Lu Cheng, Steve Roffler
Jul 28, 1999·Biochemical Pharmacology·T L ChengS R Roffler
Dec 22, 2010·Genetic Vaccines and Therapy·Sara A CollinsMark Tangney
Nov 14, 2007·PLoS Pathogens·Keith E GarrisonDouglas F Nixon
Feb 26, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Karl Erik Hellstrom, Ingegerd Hellstrom
Apr 3, 2002·Nature Medicine·Zhengmao YeKarl Erik Hellström
Mar 27, 2004·Cancer Gene Therapy·Tian-Lu ChengSteve R Roffler
Apr 20, 2006·Blood·Jessica CaprioliUNKNOWN International Registry of Recurrent and Familial HUS/TTP
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip TanJefferson Foote
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SchollerJ A Ledbetter
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A AicherE A Clark
Mar 19, 2021·Bio-protocol·Rohit Singh, Beom K Choi

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