PMID: 9435862Jan 22, 1998Paper

Costimulation of T-cell proliferation by a chimeric B7-antibody fusion protein

Cancer Immunology, Immunotherapy : CII
B GerstmayerW Wels

Abstract

T cells require at least two signals for activation and clonal expansion. The first signal conferring specificity is initiated by interaction of the T cell receptor with peptide-bearing MHC molecules. The second, costimulatory signal can be provided by cell-surface molecules on antigen-presenting cells such as B7-1 (CD80) and B7-2 (CD86), which interact with CD28 on T cells. To direct the costimulatory B7-2 molecule to the surface of tumor cells we have constructed a chimeric fusion protein, which consists of the extracellular domain of human B7-2 fused to a single-chain antibody domain (scFv) specific for the ErbB2 protein, a type I growth factor receptor overexpressed in a high percentage of human adenocarcinomas. This B7-2(225)-scFv(FRP5) molecule, expressed in the yeast Pichia pastoris and purified from culture supernatants, binds to B7 counter-receptors and to ErbB2. B7-2(225)-scFv(FRP5) localizes specifically to the surface of ErbB2-expressing target cells, thereby providing a costimulatory signal, which results in enhanced proliferation of syngeneic T cells.

Citations

Sep 25, 2002·Critical Reviews in Oncology/hematology·R S RampaulI O Ellis
Jun 27, 2006·Journal of Immunotherapy·Aihong LiuAlan L Epstein
Oct 9, 2002·The Journal of Clinical Investigation·Robin PariharWilliam E Carson
Sep 24, 1999·Biological Chemistry·R FischerN Emans
Sep 11, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Dafne Müller
Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg

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