Cotranslational assembly of protein complexes in eukaryotes revealed by ribosome profiling.

Nature
Ayala ShiberBernd Bukau

Abstract

The folding of newly synthesized proteins to the native state is a major challenge within the crowded cellular environment, as non-productive interactions can lead to misfolding, aggregation and degradation1. Cells cope with this challenge by coupling synthesis with polypeptide folding and by using molecular chaperones to safeguard folding cotranslationally2. However, although most of the cellular proteome forms oligomeric assemblies3, little is known about the final step of folding: the assembly of polypeptides into complexes. In prokaryotes, a proof-of-concept study showed that the assembly of heterodimeric luciferase is an organized cotranslational process that is facilitated by spatially confined translation of the subunits encoded on a polycistronic mRNA4. In eukaryotes, however, fundamental differences-such as the rarity of polycistronic mRNAs and different chaperone constellations-raise the question of whether assembly is also coordinated with translation. Here we provide a systematic and mechanistic analysis of the assembly of protein complexes in eukaryotes using ribosome profiling. We determined the in vivo interactions of the nascent subunits from twelve hetero-oligomeric protein complexes of Saccharomyces cerevisiae...Continue Reading

References

Nov 29, 2001·The Journal of Biological Chemistry·Hideki Sakahira, Shigekazu Nagata
Feb 15, 2002·The Journal of Biological Chemistry·Anne M SmardonPatricia M Kane
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Matthias GautschiSabine Rospert
Jun 5, 2003·Proteins·Joël JaninUNKNOWN Critical Assessment of PRedicted Interactions
Jan 24, 2006·Nature·Anne-Claude GavinGiulio Superti-Furga
Sep 1, 2006·Protein Science : a Publication of the Protein Society·Frédéric CazalsJoël Janin
Nov 3, 2007·Journal of Molecular Biology·Eleftherios KaranasiosGeorge Simos
May 20, 2008·Nature Structural & Molecular Biology·Tobias HaslbergerBernd Bukau
Aug 29, 2009·The EMBO Journal·André HalbachBernhard Dichtl
Feb 9, 2010·Nature Structural & Molecular Biology·Shashi BhushanRoland Beckmann
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Preeti GipsonWerner Kühlbrandt
Jul 14, 2010·Molecular Cell·Joris J BenschopPatrick Kemmeren
May 20, 2011·Molecular BioSystems·Bing ZhangDaniel C Liebler
Dec 7, 2011·PLoS Genetics·Caia D S Duncan, Juan Mata
Apr 16, 2013·Molecular Cell·Stefanie DuttlerJudith Frydman
Apr 12, 2014·Current Opinion in Structural Biology·Felix GlogeBernd Bukau
May 15, 2015·Briefings in Bioinformatics·Reyhaneh EsmaielbeikiCharlotte M Deane
Jul 2, 2016·Science·David BalchinF Ulrich Hartl

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Citations

Dec 7, 2018·The Journal of Biological Chemistry·Kevin C Stein, Judith Frydman
Mar 25, 2019·Bioinformatics·Reshmi RamakrishnanJoost Schymkowitz
Apr 21, 2019·Plant & Cell Physiology·Tomoya FujitaShintaro Iwasaki
Jan 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Alexander Varshavsky
May 23, 2019·Nucleic Acids Research·Anne-Sophie Gribling-BurrerSabine Rospert
Jul 25, 2019·EMBO Reports·Silke JankowskiMichael Feldbrügge
Jun 12, 2019·Molecular and Cellular Biology·Joshua J BlackArlen W Johnson
Jun 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shunnichi KashidaZoher Gueroui
Dec 18, 2019·Journal of Bacteriology·Jannes VagtsRalf Rabus
Oct 11, 2019·Nucleic Acids Research·Martine A Collart, Benjamin Weiss
Feb 12, 2020·Biomolecules·Dmitry N Ivankov, Alexei V Finkelstein
Jun 23, 2020·Annual Review of Biochemistry·Anaïs M E CassaignauJohn Christodoulou
Jul 21, 2020·PLoS Biology·Seung W RyuTanya T Paull
Jan 5, 2020·Cold Spring Harbor Symposia on Quantitative Biology·Christine Mayr
Jul 25, 2020·Genome Research·Alaaddin Bulak ArpatDavid Gatfield
Sep 13, 2019·Nature Communications·Susanne MeierMatthias J Feige
Oct 2, 2020·Physiological Reviews·Rico SchieweckMichael A Kiebler
Jan 30, 2019·Nature Structural & Molecular Biology·Olesya O PanasenkoMartine A Collart
Jul 19, 2019·Frontiers in Molecular Neuroscience·Rico Schieweck, Michael A Kiebler
Dec 7, 2018·Annual Review of Biochemistry·Günter KramerBernd Bukau
Jul 25, 2019·Nature Protocols·Carla V GalmozziGünter Kramer
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Artem MelnykovAlexander Varshavsky
Jan 16, 2020·Biomolecules·Marija LiutkuteMarina V Rodnina
Jan 23, 2020·Scientific Reports·Manuel FischerMartin Grininger
Jul 15, 2020·Nature Chemical Biology·Meta HeidenreichEmmanuel D Levy
Sep 11, 2020·Cell Communication and Signaling : CCS·Yi Liu
Nov 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Wayne S Sossin
Mar 17, 2019·The Journal of Cell Biology·Mariavittoria PizzingaMark P Ashe
May 11, 2019·Cell Stress & Chaperones·Edgar BoczekShireen Sarraf
Mar 26, 2019·Chembiochem : a European Journal of Chemical Biology·Christina S HeilMartin Grininger
Jan 28, 2020·Physical Biology·Shaked Bergman, Tamir Tuller
Sep 9, 2018·Nature·Christine Mayr
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Cheng DongJinrong Min
Jan 30, 2019·Nature Structural & Molecular Biology·Xiao-Min Liu, Shu-Bing Qian
Apr 17, 2019·Nature Communications·Ivanka KamenovaLászló Tora
Sep 19, 2020·The EMBO Journal·Konstadinos MoissogluStavroula Mili

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Datasets Mentioned

BETA
GSE116570
GSE93830

Methods Mentioned

BETA
reverse transcription PCR
PCR
protein extraction
chip
immunoprecipitation
flow cytometry

Software Mentioned

Python
FindFoci
Tophat2
LightCycler480
MetaMorph Advanced Acquisition
Metagene
SeRP
Cutadapt
Bowtie2
ImageJ

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