Cotranslational incorporation of a structurally diverse series of proline analogues in an Escherichia coli expression system

Chembiochem : a European Journal of Chemical Biology
Wookhyun KimVincent P Conticello

Abstract

A set of Escherichia coli expression strains have been defined that are competent for the incorporation of a structurally diverse series of proline analogues under culture conditions that are compatible with high levels of analogue substitution within a proline-rich protein substrate. These bacterial strains have been employed to assay the efficacy of incorporation of noncanonical amino acids into a recombinant-protein test substrate and to create variant polypeptides in which native protein sequences have been globally substituted with imino acid analogues in response to proline codons. We envision that these methods may be used to interrogate the effect of imino acid substitution on protein structure and function and may be particularly informative in the context of structural comparison of a series of modified proteins with respect to the stereoelectronic differences between the incorporated proline analogues.

References

Mar 20, 1991·Journal of Molecular Biology·M W MacArthur, J M Thornton
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P H HirelS Blanquet
Sep 5, 1988·Journal of Molecular Biology·C M Wilmot, J M Thornton
Apr 1, 1986·Journal of Bacteriology·S GrotheJ M Wood
Jan 1, 1980·Molecular & General Genetics : MGG·K Bohman, L A Isaksson
Dec 1, 1994·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Sep 1, 1994·International Journal of Peptide and Protein Research·N PanasikR T Raines
Jan 15, 1994·The Biochemical Journal·M P Williamson
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·B Brodsky, J A Ramshaw
Feb 12, 1998·Journal of Molecular Biology·J Prieto, L Serrano
May 16, 1998·Nature·S K HolmgrenR T Raines
Jun 27, 1998·Journal of Molecular Biology·U ReimerG Fischer
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N BudisaR Huber
Feb 18, 1999·Chemistry & Biology·S K HolmgrenR T Raines
Sep 24, 1999·Protein Science : a Publication of the Protein Society·A R Viguera, L Serrano
Dec 3, 1999·Science·M Ibba, D Söll
Sep 1, 2000·Journal of Molecular Biology·S J Eyles, L M Gierasch
Jan 12, 2001·Journal of Molecular Biology·B LiV Daggett
Jul 18, 2001·Journal of the American Chemical Society·L E BretscherR T Raines
Nov 10, 2001·Trends in Biochemical Sciences·H Reiersen, A R Rees
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Kristi L KiickCarolyn R Bertozzi
Mar 29, 2002·Chembiochem : a European Journal of Chemical Biology·Kent KirshenbaumDavid A Tirrell
May 16, 2002·Journal of the American Chemical Society·Deepshikha DattaDavid A Tirrell
Jul 18, 2002·Chemical Communications : Chem Comm·Lei Wang, Peter G Schultz
Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Oct 26, 2002·The Journal of Biological Chemistry·Douglas D BuechterElliott A Gruskin
Nov 6, 2002·Journal of Molecular Biology·Frank S CordesMark S P Sansom
Dec 12, 2002·Biochemistry·William J WedemeyerHarold A Scheraga
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Satwik KamtekarThomas A Steitz

❮ Previous
Next ❯

Citations

Aug 16, 2008·Biochemistry·Kelly L GorresRonald T Raines
Oct 6, 2009·The Journal of Organic Chemistry·Grant R KrowRonald T Raines
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew D ShouldersRonald T Raines
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·A James LinkDavid A Tirrell
Apr 20, 2010·Organic & Biomolecular Chemistry·Jari J KoivistoAri M P Koskinen
Jul 1, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sarah R MacEwan, Ashutosh Chilkoti
Jan 6, 2009·Materials Science & Engineering. R, Reports : a Review Journal·Dominic ChowAshutosh Chilkoti
Jun 8, 2006·Chembiochem : a European Journal of Chemical Biology·Soojin SonDavid A Tirrell
Apr 30, 2013·Chembiochem : a European Journal of Chemical Biology·I-Lin WuVincent P Conticello
Nov 25, 2011·Chembiochem : a European Journal of Chemical Biology·Hidetoshi TeramotoJun Ishibashi
Sep 19, 2015·Chembiochem : a European Journal of Chemical Biology·Daniel RodererMarina Rubini
May 1, 2012·Biochemical and Biophysical Research Communications·Maud LarregolaNediljko Budisa
Aug 4, 2005·Methods : a Companion to Methods in Enzymology·A James Link, David A Tirrell
Jan 7, 2006·Chembiochem : a European Journal of Chemical Biology·Marissa L MockDavid A Tirrell
Dec 22, 2009·Molecular BioSystems·Natalya Voloshchuk, Jin Kim Montclare
Jun 10, 2017·Journal of the American Chemical Society·Seth A LieblichDavid A Tirrell
Jan 19, 2020·Biophysics Reviews·Himal Kanti Ganguly, Gautam Basu
Mar 18, 2021·Beilstein Journal of Organic Chemistry·Vladimir KubyshkinNediljko Budisa
Apr 1, 2007·EcoSal Plus·Laszlo N Csonka, Thomas Leisinger
Aug 21, 2021·Chembiochem : a European Journal of Chemical Biology·Matthew C T Hartman
Sep 22, 2021·Chembiochem : a European Journal of Chemical Biology·Jennie O' LoughlinMarina Rubini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.