Cotton GhMYB7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic Arabidopsis

Science China. Life Sciences
Junfeng HuangWenliang Xu

Abstract

The secondary cell wall in mature cotton fibers contains over 90% cellulose with low quantities of xylan and lignin. However, little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers. In this study, we characterized an R2R3-MYB transcription factor, GhMYB7, in cotton. GhMYB7 is expressed at a high level in developing fibers and encodes a MYB protein that is targeted to the cell nucleus and has transcriptional activation activity. Ectopic expression of GhMYB7 in Arabidopsis resulted in small, curled, dark green leaves and also led to shorter inflorescence stems. A cross-sectional assay of basal stems revealed that cell wall thickness of vessels and interfascicular fibers was higher in transgenic lines overexpressing GhMYB7 than in the wild type. Constitutive expression of GhMYB7 in Arabidopsis activated the expression of a suite of secondary cell wall biosynthesis-related genes (including some secondary cell wall-associated transcription factors), leading to the ectopic deposition of cellulose and lignin. The ectopic deposition of secondary cell walls may have been initiated before the cessation of cell expansion. Moreover, GhMYB7 was capable of binding to the promoter regions of AtSND1 and A...Continue Reading

References

Feb 27, 2003·Plant Molecular Biology·M L CedroniJ F Wendel
Mar 25, 2009·The Plant Journal : for Cell and Molecular Biology·Adriane MachadoElizabeth S Dennis
Dec 8, 2009·Plant Physiology·Charles T AndersonChris Somerville
Jan 14, 2011·Trends in Plant Science·Qiao Zhao, Richard A Dixon
Jan 18, 2011·The Plant Journal : for Cell and Molecular Biology·Sally-Ann WalfordElizabeth S Dennis
Jun 5, 2012·Frontiers in Plant Science·Candace H HaiglerJohn R Tuttle
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Sep 13, 2012·The Plant Journal : for Cell and Molecular Biology·David ÖhmanBjörn Sundberg
Apr 30, 2013·Plant Molecular Biology·Wen-Liang XuXue-Bao Li
Jun 20, 2013·Frontiers in Plant Science·Hua Cassan-WangJacqueline Grima-Pettenati
Oct 9, 2014·Plant & Cell Physiology·Ruiqin Zhong, Zheng-Hua Ye
Jan 1, 2013·The Plant Journal : for Cell and Molecular Biology·Won-Chan KimKyung-Hwan Han

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Citations

Feb 2, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaomei FangZhengsheng Zhang
Dec 30, 2017·Science China. Life Sciences·Fuguang LiZhao'en Yang
Nov 29, 2017·International Journal of Genomics·Bahaeldeen Babikar MohamedTayyab Husnain
Jun 24, 2018·Science China. Life Sciences·Xuan Ma, Xiaofeng Cao

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