Could a plant derived protein potentiate the anticancer effects of a stem cell in brain cancer?

Oncotarget
Camila Ramalho BonturiMaria Luiza Vilela Oliva

Abstract

Glioblastoma is the most aggressive brain tumor with poor overall survival bellow 2 years. The natural compounds with anti-cancer properties, are thus gaining attention for possible adjuvant GBM treatment. In various cancer models Enterolobium contortisiliquum Trypsin Inhibitor (EcTI) proved to have anti-cancer effects. Here, we investigated the EcTI effects on GBM U87 cells and on mesenchymal stem cells (MSC) compared to their direct coculture (MSC/U87). MSC are present in tumor stroma, modulating GBM cells phenotype, and also represent potential drug delivery vehicle due to their tumor tropism. We showed that in p53-wild type U87 cells, metabolic activity was less affected by EcTI as in MSC monocuture, but the metabolic rate of mixed coculture was significantly reduced at lower EcTI concentration. Under coculture condition, EcTI potentiated MSC induced cell cycle arrest, possible due to highly increased p53, p21 and lower D1 expression, but there was no effect on apoptosis. Accordingly, in the coculture EcTI also enhanced Ca2+ signalling mediated via bradykinin receptor 2, being associated with nitric oxide release that highly impaired proliferation and invasion. The mechanism did not seem to involve changes in cell adhesion ...Continue Reading

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Citations

Jun 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camila Ramalho BonturiMaria Luiza Vilela Oliva
Nov 17, 2020·Protein and Peptide Letters·Leydianne Leite de Siqueira PatriotaThiago Henrique Napoleáo

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Methods Mentioned

BETA
size exclusion chromatography
reversed-phase chromatography
electrophoresis
FACS
Fluorescence
PCR

Software Mentioned

Leica Application Suite EZ
GelCapture
FlexStation
LuminexMAP
Flow Jo 9
SDS
Image J

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