Could the cytokeratin molecule be modulated during tumor transformation in hepatocellular carcinoma?

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
B SuY Lai

Abstract

The stability of cytokeratin during tumor transformation in hepatocellular carcinoma was studied. We applied biochemical methodology to look into the switching of cytokeratin molecules in tumor transformation. First, by centrifugation the cytokeratin molecules were extracted from both liver and hepatoma tissues. The extracts were then soaked with cyanogen bromide-activated Sepharose 4B beads previously coated by monoclonal anti-cytokeratin antibody. The bound molecules were then released from the resin with salt. Second, the isolated molecules of both were treated with lysosomal enzyme and analyzed on two-dimensional gels. The results demonstrated that there was a modulation in cytokeratin molecules, and the hepatoma cytokeratin was generated from the hepatocyte cytokeratin.

Citations

Mar 23, 2011·Medical Molecular Morphology·Yi-Hsiang LiuYih-Shyong Lai
Nov 27, 2007·Journal of Molecular Histology·Chiung-Chi ChengYih-Shyong Lai
Mar 22, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y LiuY Lai
Mar 23, 2011·Biochemical and Biophysical Research Communications·Yi-Hsiang LiuYih-Shyong Lai
Dec 15, 2010·Biochemical and Biophysical Research Communications·Yi-Hsiang LiuChin-Chin Ho

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