Coulometric calcium pump for thin layer sample titrations

Analytical Chemistry
Majid Ghahraman AfsharEric Bakker

Abstract

A selective electrochemical calcium pump based on a fast diffusive calcium ionophore-based membrane is reported. An initially nonpolarized ionophore-based membrane allows one to establish a net calcium flux by applying a potential step function (i.e., 250 mV for 30 s). The resulting calcium flux is released into a microliter scale thin layer reservoir, and the resulting ion perturbation is monitored by either a potentiometric or a coulometric readout. This chemical perturbation in the thin layer thus acts as a titration agent that is precisely controlled by coulometry. A linear correlation between released and detected calcium is confirmed by the two different readout modes. Having demonstrated the efficiency of the calcium pump in background electrolyte solutions, a complexometric titration with known concentrations of EDTA in the thin layer sample was performed. With the potentiometric readout, titrations in the range of 0.25-0.75 mM gave a precision of 3%, whereas the coulometric readout gave a range of 0.02-0.12 mM and a precision of 2%. Improved precision is expected by better control of the thin layer geometry by microfabrication. The significance of this work is that the coupling of a selective calcium pump with a thin l...Continue Reading

References

Feb 20, 2002·Chemical Reviews·Eric BakkerErnö Pretsch
Apr 2, 2002·Analytical Chemistry·Shigeo SawadaToshiyuki Osakai
Nov 25, 2003·Analytical Chemistry·Alexey Shvarev, Eric Bakker
Sep 30, 2009·Journal of the American Chemical Society·Jiawang Ding, Wei Qin
May 1, 2010·Analytical Chemistry·Ewa Grygolowicz-Pawlak, Eric Bakker
Aug 17, 2010·Electrochemistry Communications·Ewa Grygolowicz-Pawlak, Eric Bakker
Aug 25, 2012·Analytical Chemistry·Alexey ShvarevEric Bakker
Sep 1, 2012·Analytica Chimica Acta·Manzar SohailEric Bakker
Nov 6, 2012·Angewandte Chemie·Gastón A CrespoEric Bakker
Nov 13, 2012·Analytical Chemistry·Gastón A CrespoEric Bakker
Mar 22, 2013·Analytical Chemistry·Xu U Zou, Philippe Bühlmann
May 30, 2014·Analytical Chemistry·Zdeňka JarolímováEric Bakker
Oct 16, 2014·Analytical Chemistry·Maria CuarteroEric Bakker
Jan 8, 2015·Analytical Chemistry·Maria CuarteroEric Bakker
Dec 17, 2015·Chimia·Maria CuarteroEric Bakker

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Citations

Nov 6, 2015·Analytical Chemistry·Eric Bakker
Apr 10, 2018·Journal of Analytical Methods in Chemistry·Samuel MelakuRajeev B Dabke
Apr 25, 2019·Accounts of Chemical Research·Elena Zdrachek, Eric Bakker
Jul 21, 2017·ACS Sensors·Jingying ZhaiEric Bakker
May 29, 2018·Analytical Chemistry·Elena Zdrachek, Eric Bakker

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